IRF3 Antikörper (AA 1-419)

Produktnummer ABIN8001197

Dieser Maus Monoklonal Antikörper detektiert spezifisch IRF3 in WB, IF, FACS und ICC. Es zeigt Reaktivität gegenüber Proben von Maus und Ratte.

Kurzübersicht für IRF3 Antikörper (AA 1-419) (ABIN8001197)

Target: IRF3 (Interferon Regulatory Factor 3 (IRF3))

Reaktivität: Maus, Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser IRF3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Güteklasse: Carrier-free

Klon: 3B4

Produktdetails anti-IRF3 Antikörper

Bindungsspezifität: AA 1-419

Verwendungszweck: Anti-IRF3 Antibody (monoclonal, 3B4)

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-IRF3 Antibody.(monoclonal, 3B4)-3. Tested in Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Mouse, Rat.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived mouse IRF3 recombinant protein (Position: M1-I419).

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: Western blot, 0.25-0.5 μg/mL, Mouse, Rat Immunocytochemistry/Immunofluorescence, 5 μg/mL, Mouse Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Mouse

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Haltbarkeit: 12 months

Details zu IRF3

Target: IRF3 (Interferon Regulatory Factor 3 (IRF3))

Andere Bezeichnung: Irf3

Hintergrund:

Background: IRF3 (interferon regulatory factor 3) is a member of the interferon regulatory transcription factor (IRF) family. The IRF3 gene is mapped on 19q13.33. IRF3 is found in an inactive cytoplasmic form that upon serine/threonine phosphorylation forms a complex with CREBBP. IRF3 plays an important role in the innate immune system's response to viral infection. Aggregated MAVS have been found to activate IRF3 dimerization. Although IRF3 increased transcriptional activity from an ISRE-containing promoter, expression of IRF3 as a Gal4 fusion protein did not activate expression of a chloramphenicol acetyltransferase (CAT) < a href=https://www.bosterbio.com/cells/reporter-cell-lines style=color:#ea8d28>reporter gene containing repeats of the Gal4-binding sites. Translocation of IRF3 was accompanied by an increase in serine and threonine phosphorylation. The transcriptional activators CREBBP and EP300 coimmunoprecipitated with IRF3 only subsequent to viral infection, and the authors stated that these are also subunits of DRAF1.

Gene Full Name: interferon regulatory factor 3

Molekulargewicht: 50-55 kDa

Gen-ID: 54131

UniProt: P70671

Pathways: TLR Signalweg, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Toll-Like Receptors Cascades