BTK Antikörper (AA 581-659)

Produktnummer ABIN750098

Der Kaninchen Polyklonal Anti-BTK-Antikörper wurde für WB, ELISA, IF (cc), IF (p) und IHC (fro) validiert. Er ist geeignet, BTK in Proben von Ratte zu detektieren. Es ist 1 Publikation verfügbar.

Kurzübersicht für BTK Antikörper (AA 581-659) (ABIN750098)

Target: BTK (Bruton Agammaglobulinemia tyrosine Kinase (BTK))

Reaktivität: Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser BTK Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Produktdetails anti-BTK Antikörper

Bindungsspezifität: AA 581-659

Kreuzreaktivität: Ratte

Homologie: Human,Mouse,Dog,Cow,Pig,Horse,Rabbit

Aufreinigung: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human BTK

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB 1:300-5000
ELISA 1:500-1000
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Haltbarkeit: 12 months

Referenzen für anti-BTK Antikörper (ABIN750098)

Liu, Zhang, Han, Wang, Liu, Zhang, Zhou, Xiang: "Inhibition of BTK protects lungs from trauma-hemorrhagic shock-induced injury in rats." in: Molecular medicine reports, Vol. 16, Issue 1, pp. 192-200, (2017) (PubMed).

Details zu BTK

Target: BTK (Bruton Agammaglobulinemia tyrosine Kinase (BTK))

Andere Bezeichnung: BTK

Hintergrund:

Synonyms: Agammaglobulinaemia tyrosine kinase, AGMX 1, AGMX1, AT, ATK, B cell progenitor kinase, B-cell progenitor kinase, BPK, Bruton agammaglobulinemia tyrosine kinase, Bruton tyrosine kinase, Bruton?s Tyrosine Kinase, Btk, BTK_HUMAN, IMD 1, IMD1, MGC126261, MGC126262, OTTHUMP00000063593, PSCTK 1, PSCTK1, Tyrosine protein kinase BTK, Tyrosine-protein kinase BTK, XLA.

Background: Brutons tyrosine kinase (BTK) is a member of the BTK/Tec family of cytoplasmic tyrosine kinases. Like other BTK family members, it contains a pleckstrin homology (PH) domain, Src homology SH3 and SH2 domains. BTK plays an important role in B cell development. Activation of B cells by various ligands is accompanied by BTK membrane translocation mediated by its PH domain binding to phosphatidylinositol-3,4,5-trisphosphate. The membrane located BTK is active and associated with transient phosphorylation of two tyrosine residues, Tyr551 and Tyr223. Tyr551 in the activation loop is transphosphorylated by the Src family tyrosine kinase, leading to autophosphorylation at Tyr223 within the SH3 domain, which is necessary for full activation. The activation of BTK is negatively regulated by PKC beta through phosphorylation of BTK at Ser180, which results in reduced membrane recruitment, transphosphorylation and subsequent activation. The PKC/BTK inhibitory signal is likely to be a key determinant of the B cell receptor signaling threshold to maintain optimal BTK activity.

Gen-ID: 695

Pathways: Fc-epsilon Rezeptor Signalübertragung, Hormone Transport, Activation of Innate immune Response, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, Toll-Like Receptors Cascades, BCR Signaling