GDF15 Antikörper (AA 51-199)

Produktnummer ABIN7995316

Der Kaninchen Polyklonal anti-GDF15 Antikörper wird verwendet zum Nachweis von GDF15 in Proben von Human, Maus und Ratte. Er wurde validiert für WB, IHC, ELISA, FACS und IF.

Kurzübersicht für GDF15 Antikörper (AA 51-199) (ABIN7995316)

Target: GDF15 (Growth Differentiation Factor 15 (GDF15))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser GDF15 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS), Immunofluorescence (IF)

Güteklasse: Carrier-free

Produktdetails anti-GDF15 Antikörper

Bindungsspezifität: AA 51-199

Verwendungszweck: Anti-PDF Antibody

Kreuzreaktivität (Details): No cross reactivity with other proteins.

Homologie: Human PDF shares 82.4% amino acid (aa) sequence identity with mouse PDF.

Produktmerkmale: Anti-PDF Antibody. Tested in ELISA, IF, IHC, WB, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human PDF recombinant protein (Position: H51-Q199). Human PDF shares 82.4% amino acid (aa) sequence identity with mouse PDF.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human Immunofluorescence, 5 μg/mL, Human Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human ELISA, 0.1-0.5 μg/mL, -

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Haltbarkeit: 12 months

Details zu GDF15

Target: GDF15 (Growth Differentiation Factor 15 (GDF15))

Andere Bezeichnung: PDF

Hintergrund:

Background: Protein synthesis proceeds after formylation of methionine by methionyl-tRNA formyl transferase (FMT) and transfer of the charged initiator f-met tRNA to the ribosome. In eubacteria and eukaryotic organelles the product of this gene, peptide deformylase (PDF), removes the formyl group from the initiating methionine of nascent peptides. In eubacteria, deformylation of nascent peptides is required for subsequent cleavage of initiating methionines by methionine aminopeptidase. The discovery that a natural inhibitor of PDF, actinonin, acts as an antimicrobial agent in some bacteria has spurred intensive research into the design of bacterial-specific PDF inhibitors. In human cells, only mitochondrial proteins have N-formylation of initiating methionines. Protein inhibitors of PDF or siRNAs of PDF block the growth of cancer cell lines but have no effect on normal cell growth. In humans, PDF function may therefore be restricted to rapidly growing cells.

Gene Full Name: peptide deformylase, mitochondrial

Molekulargewicht: 25 kDa

Gen-ID: 64146

UniProt: Q9HBH1

Pathways: SARS-CoV-2 Protein Interaktom