GDF15 Antikörper (Growth Differentiation Factor 15) (C-Term) (HRP)

Details for Product anti-GDF15 Antibody No. ABIN1043915, Anbieter: Anmelden zum Anzeigen
Antigen
  • GDF15
  • GDF-15
  • MIC-1
  • MIC1
  • NAG-1
  • PDF
  • PLAB
  • PTGFB
  • SBF
  • growth differentiation factor 15
  • GDF15
  • Gdf15
Epitop
C-Term
58
46
17
15
14
12
11
8
6
5
5
4
3
3
3
2
2
2
2
2
1
1
1
1
1
1
Reaktivität
Human, Maus
289
81
41
4
4
3
3
Wirt
Kaninchen
220
66
20
7
Klonalität
Polyklonal
Konjugat
Dieser GDF15 Antikörper ist konjugiert mit HRP
37
28
13
7
7
5
3
3
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Applikation
ELISA, Western Blotting (WB)
259
206
73
59
28
13
10
7
5
4
1
1
1
1
Optionen
Hersteller
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Hersteller Produkt- Nr.
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Immunogen Anti-NAG-1 (C-terminal specific) antibody was prepared by repeated immunizations with a synthetic peptide corresponding to a region near the carboxy terminal end of human NAG-1 protein.  A residue of cysteine was added to facilitate coupling to KLH.
Immunogen Type: Peptide
Isotyp IgG
Spezifität Anti-NAG-1 (C-terminal specific) was affinity purified from monospecific antiserum by immunoaffinity chromatography.  This antibody reacts with the C-terminus of endogenous NAG-1 protein from human and mouse tissues.  A BLAST analysis suggests reactivity with NAG-1 from chimpanzee and macaque based on a 100% homology. Partial reactivity is expected against rat based on an 86% homology with the immunizing sequence.  Cross-reactivity with NAG-1 from other sources has not been determined.
Produktmerkmale Non-steroidal anti-inflammatory drug (NSAID) activated gene (NAG-1) is a member of the transforming growth factor-beta (TGF-beta) superfamily. NAG-1 is also known as Macrophage Inhibitory Cytokine-1 (MIC-1), Growth Differentiation Factor 15 (GDF15), Placental Bone Morphogenetic Protein (PLAB), or Prostate Derived Factor (PDF). NAG-1 is expressed in human placenta, prostate and colon. It possesses antitumorigenic and proapoptotic activities. NAG-1 expression is dramatically increased in inflammation, injury and malignancy. Increase of NAG-1 expression is a feature of many cancers including breast, colon, pancreas and prostate. In a number of studies, NAG-1 expression was increased by a number of NSAIDs. This increase in expression may correlate with the chemopreventive effect NSAIDs seem to have with certain cancers. NAG-1 expression is also induced by PPAR gamma ligands and by several dietary compounds such as conjugated linoleic acids (CLAs), naturally occurring fatty acids in ruminant food products, indoles, epicatechin gallate, and genistein. Induced expression of NAG-1 results in stimulation of apoptosis and inhibition of cell growth. Inhibition of NAG-1 induced expression by small interference RNA (siRNA) results in repression of induced apoptosis. NAG-1 expression is regulated by a numbers of transcription factors such as ERG-1 and Sp1. EGR-1 may be necessary for NSAID-induced NAG-1 expression. The study of expression of NAG-1 proteins, including variants, is important to define their potential role as serum biomarkers for cancer diagnosis, treatment monitoring, epidemiology study, and nutrition surveys.
Andere Bezeichnung Nag-1 (GDF15 Antibody Abstract)
Hintergrund Non-steroidal anti-inflammatory drug (NSAID) activated gene (NAG-1) is a member of the transforming growth factor-beta (TGF-beta) superfamily. NAG-1 is also known as Macrophage Inhibitory Cytokine-1 (MIC-1), Growth Differentiation Factor 15 (GDF15), Placental Bone Morphogenetic Protein (PLAB), or Prostate Derived Factor (PDF). NAG-1 is expressed in human placenta, prostate and colon. It possesses antitumorigenic and proapoptotic activities. NAG-1 expression is dramatically increased in inflammation, injury and malignancy. Increase of NAG-1 expression is a feature of many cancers including breast, colon, pancreas and prostate. In a number of studies, NAG-1 expression was increased by a number of NSAIDs. This increase in expression may correlate with the chemopreventive effect NSAIDs seem to have with certain cancers. NAG-1 expression is also induced by PPAR gamma ligands and by several dietary compounds such as conjugated linoleic acids (CLAs), naturally occurring fatty acids in ruminant food products, indoles, epicatechin gallate, and genistein. Induced expression of NAG-1 results in stimulation of apoptosis and inhibition of cell growth. Inhibition of NAG-1 induced expression by small interference RNA (siRNA) results in repression of induced apoptosis. NAG-1 expression is regulated by a numbers of transcription factors such as ERG-1 and Sp1. EGR-1 may be necessary for NSAID-induced NAG-1 expression. The study of expression of NAG-1 proteins, including variants, is important to define their potential role as serum biomarkers for cancer diagnosis, treatment monitoring, epidemiology study, and nutrition surveys.
Synonyms: NAG-1, GDF15, MIC-1, nonsteroidal anti-inflammatory drug-activated gene, NSAID-activated gene 1 protein, growth differentiation factor 15, macrophage inhibitory compound 1, prostate-derived factor
Gen-ID 9518
UniProt Q99988
Applikationshinweise Anti-NAG-1 (C-terminal specific) antibody is suitable for ELISA and western blotting of human and mouse NAG-1 protein.  For detection of NAG-1 in human serum, a sandwich ELISA is suggested using this antibody in combination with anti-NAG-1/GDF15 (N-terminal), H variant or D variant specific antibodies.  Specific conditions for reactivity should be optimized by the end user.  Expect bands in Western blots of approximately 14 and 28 kDa in size corresponding to NAG-1 monomer and dimer, respectively, using the appropriate cell lysate or extract.
Kommentare

Gene Name: GDF15

Beschränkungen Nur für Forschungszwecke einsetzbar
Format Lyophilized
Rekonstitution Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 100 µL
Konzentration 1.07 mg/ml
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Konservierungsmittel Gentamicin sulfate
Handhabung Do NOT add Sodium Azide!
Lagerung 4 °C/-20 °C
Informationen zur Lagerung Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -20 °C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.
Haltbarkeit 12 months
Bilder des Herstellers
Western Blotting (WB) image for anti-Growth Differentiation Factor 15 (GDF15) (C-Term) antibody (HRP) (ABIN1043915) Western blot using affinity purified anti-NAG-1/GDF15 (C-terminal) antibody shows det...
 image for anti-Growth Differentiation Factor 15 (GDF15) (C-Term) antibody (HRP) (ABIN1043915) anti-Growth Differentiation Factor 15 (GDF15) (C-Term) antibody (HRP) (Image 2)
 image for anti-Growth Differentiation Factor 15 (GDF15) (C-Term) antibody (HRP) (ABIN1043915) anti-Growth Differentiation Factor 15 (GDF15) (C-Term) antibody (HRP) (Image 3)
Western Blotting (WB) image for anti-Growth Differentiation Factor 15 (GDF15) (C-Term) antibody (HRP) (ABIN1043915) Western blot using affinity purified anti-NAG-1/GDF15 (C-terminal) antibody shows de...
Immunohistochemistry (IHC) image for anti-Growth Differentiation Factor 15 (GDF15) (C-Term) antibody (HRP) (ABIN1043915) Immunohistochemistry of Rabbit anti NAG1 antibody (C-terminal specific) at 20X in col...
Immunohistochemistry (IHC) image for anti-Growth Differentiation Factor 15 (GDF15) (C-Term) antibody (HRP) (ABIN1043915) Immunohistochemistry of Rabbit anti NAG1 antibody (C-terminal specific) at 20X in col...
Produkt verwendet in: Baek, Eling: "Changes in gene expression contribute to cancer prevention by COX inhibitors." in: Progress in lipid research, Vol. 45, Issue 1, pp. 1-16, 2006 (PubMed).

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