Dynactin 1 Antikörper (AA 1042-1278)

Produktnummer ABIN7986532

Der Kaninchen Polyklonal anti-Dynactin 1 Antikörper wird verwendet zum Nachweis von Dynactin 1 in Proben von Human, Maus und Ratte. Er wurde validiert für WB, ELISA, IHC, IF, FACS und ICC.

Kurzübersicht für Dynactin 1 Antikörper (AA 1042-1278) (ABIN7986532)

Target: Dynactin 1 (DCTN1)

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Dynactin 1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Güteklasse: Carrier-free

Produktdetails anti-Dynactin 1 Antikörper

Bindungsspezifität: AA 1042-1278

Verwendungszweck: Anti-DCTN1/p150-glued Antibody

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-DCTN1/p150-glued Antibody. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human DCTN1/p150-glued recombinant protein (Position: K1042-S1278).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.1-0.25 μg/mL, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human ELISA, 0.1-0.5 μg/mL, -

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Haltbarkeit: 12 months

Details zu Dynactin 1

Target: Dynactin 1 (DCTN1)

Andere Bezeichnung: DCTN1

Hintergrund:

Background: Dynactin subunit 1 is a protein that in humans is encoded by the DCTN1 gene. This gene encodes the largest subunit of dynactin, a macromolecular complex consisting of 10 subunits ranging in size from 22 to 150 kD. Dynactin binds to both microtubules and cytoplasmic dynein. Dynactin is involved in a diverse array of cellular functions, including ER-to-Golgi transport, the centripetal movement of lysosomes and endosomes, spindle formation, chromosome movement, nuclear positioning, and axonogenesis. This subunit interacts with dynein intermediate chain by its domains ly binding to dynein and binds to microtubules via a highly conserved glycine-rich cytoskeleton-associated protein (CAP-Gly) domain in its N-terminus. Alternative splicing of this gene results in multiple transcript variants encoding distinct isoforms. Mutations in this gene cause distal hereditary motor neuronopathy type VIIB (HMN7B) which is also known as distal spinal and bulbar muscular atrophy (dSBMA).

Gene Full Name: dynactin subunit 1

Molekulargewicht: 150 kDa

Gen-ID: 1639

UniProt: Q14203

Pathways: M Phase, ER-Nucleus Signaling