Cyclin B1 Antikörper (AA 1-433)

Produktnummer ABIN7982131

Der Kaninchen Polyklonal anti-Cyclin B1 Antikörper wird verwendet zum Nachweis von Cyclin B1 in Proben von Human. Er wurde validiert für WB, IF, FACS und ICC.

Kurzübersicht für Cyclin B1 Antikörper (AA 1-433) (ABIN7982131)

Target: Cyclin B1 (CCNB1)

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Cyclin B1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Güteklasse: Carrier-free

Produktdetails anti-Cyclin B1 Antikörper

Bindungsspezifität: AA 1-433

Verwendungszweck: Anti-Cyclin B1/CCNB1 Antibody

Spezifität: No cross reactivity with other proteins.

Kreuzreaktivität (Details): No cross-reactivity with other proteins

Homologie: Human Cyclin B1 shares 86%,85% amino acid (aa) sequences identity with mouse,rat Cyclin B1,respectively.

Produktmerkmale: Anti-Cyclin B1/CCNB1 Antibody. Tested in Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human Cyclin B1 recombinant protein (Position: M1-V433). Human Cyclin B1 shares 86% and 85% amino acid (aa) sequences identity with mouse and rat Cyclin B1, respectively.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.1-0.5 μg/mL, Human Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg Sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Haltbarkeit: 12 months

Details zu Cyclin B1

Target: Cyclin B1 (CCNB1)

Andere Bezeichnung: CCNB

Hintergrund:

Background: CCNB also known as Cyclin B1, is a protein that in humans is encoded by the CCNB1 gene, and it is mapped to 5q13.2. The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product complexes with p34 (cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites. CCNB contributes to the switch-like all or none behavior of the cell in deciding to commit to mitosis. Its activation is well-regulated, and positive feedback loops ensure that once the cyclin B1-Cdk1 complex is activated, it is not deactivated.

Gene Full Name: cyclin B1

Sequence Similarities: Belongs to the cyclin family. Cyclin AB subfamily.

Molekulargewicht: 56 kDa

Gen-ID: 891

UniProt: P14635

Pathways: Zellzyklus, AMPK Signaling, Mitotic G1-G1/S Phases, M Phase