Aurora A Antikörper

Produktnummer ABIN7938052

Der Maus Monoklonal anti-Aurora A Antikörper (Klon 35C1) (ABIN7938052) detektiert spezifisch Aurora A in WB, IF, ICC, IP und FACS. Dieser Antikörper reagiert spezifisch mit Proben aus Human.

Kurzübersicht für Aurora A Antikörper (ABIN7938052)

Target: Aurora A (AURKA) (Aurora Kinase A (AURKA))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Aurora A Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunoprecipitation (IP), Flow Cytometry (FACS)

Klon: 35C1

Produktdetails anti-Aurora A Antikörper

Verwendungszweck: Aurora A antibody (mAb) (Clone 35C1)

Aufreinigung: Protein A Chromatography

Immunogen: This antibody was raised against full-length recombinant human Aurora A protein.

Isotyp: IgG2b

Anwendungsinformationen

Applikationshinweise: WB: 1 - 2 μg/mL dilution The addition of 0.05 % Tween 20 in the blocking buffer and primary antibody incubation buffer is recommended to aid in detection by Western blot. Individual optimization may be required.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: Purified IgG in PBS with 30 % glycerol and 0.035 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Lagerung: -20 °C

Informationen zur Lagerung: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Haltbarkeit: 24 months

Details zu Aurora A

Target: Aurora A (AURKA) (Aurora Kinase A (AURKA))

Andere Bezeichnung: Aurora A

Hintergrund:

Background_Plain: Aurora A (Aurora Kinase A) is a mitotic serine/threonine kinases that contributes to the regulation of cell cycle progression. Associates with the centrosome and the spindle microtubules during mitosis and plays a critical role in various mitotic events including the establishment of mitotic spindle, centrosome duplication, centrosome separation as well as maturation, chromosomal alignment, spindle assembly checkpoint, and cytokinesis. Required for initial activation of CDK1 at centrosomes. Phosphorylates numerous target proteins, including ARHGEF2, BORA, BRCA1, CDC25B, DLGP5, HDAC6, KIF2A, LATS2, NDEL1, PARD3, PPP1R2, PLK1, RASSF1, TACC3, p53/TP53 and TPX2. Regulates KIF2A tubulin depolymerase activity. Required for normal axon formation. Plays a role in microtubule remodeling during neurite extension. Important for microtubule formation and/or stabilization. Also acts as a key regulatory component of the p53/TP53 pathway, and particularly the checkpoint-response pathways critical for oncogenic transformation of cells, by phosphorylating and stabilizing p53/TP53. Phosphorylates its own inhibitors, the protein phosphatase type 1 (PP1) isoforms, to inhibit their activity. Necessary for proper cilia disassembly prior to mitosis.

Aliases: AIK, ARK1, AURA, BTAK, STK6, STK7, STK15, AURORA2, PPP1R47

Molekulargewicht: 50 kDa

NCBI Accession: NP_003591

Pathways: Zellzyklus, Asymmetric Protein Localization