ENOS Antikörper (pSer1177)

Produktnummer ABIN746468

Der Kaninchen Polyklonal Anti-ENOS-Antikörper wurde für WB, ELISA, IF (cc), IF (p), FACS, IHC (p) und IHC (fro) validiert. Er ist geeignet, ENOS in Proben von Human, Ratte und Maus zu detektieren. Es sind 4+ Publikationen verfügbar.

Kurzübersicht für ENOS Antikörper (pSer1177) (ABIN746468)

Target: ENOS (NOS3) (Nitric Oxide Synthase 3 (Endothelial Cell) (NOS3))

Reaktivität: Human, Ratte, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser ENOS Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Produktdetails anti-ENOS Antikörper

Bindungsspezifität: pSer1177

Kreuzreaktivität: Human, Maus, Ratte

Homologie: Dog,Cow,Sheep,Pig,Rabbit,Guinea Pig

Aufreinigung: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human eNOS around the phosphorylation site of Ser1177

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Haltbarkeit: 12 months

Referenzen für anti-ENOS Antikörper (ABIN746468)

Chen, Tan, Wu, Chai, Yeh, Chou, Chen, Dai: "Statins ameliorate pulmonary hypertension secondary to left ventricular dysfunction through the Rho-kinase pathway and NADPH oxidase." in: Pediatric pulmonology, Vol. 52, Issue 4, pp. 443-457, (2016) (PubMed).

Yin, Wan, Li, Jia, Sun, Pan, Wan: "Protective effect of Xin Mai Jia ultrafiltration extract on human umbilical vein endothelial cell injury induced by hydrogen peroxide and the effect on the NO-cGMP signaling pathway." in: Experimental and therapeutic medicine, Vol. 8, Issue 1, pp. 38-48, (2014) (PubMed).

Ikemura, Yamamoto, Motomura, Yamaguchi, Zhao, Iwasaki, Iwamoto: "Preventive effects of the anti-vasospasm agent via the regulation of the Rho-kinase pathway on the development of steroid-induced osteonecrosis in rabbits." in: Bone, Vol. 53, Issue 2, pp. 329-35, (2013) (PubMed).

Chuang, Lin, Huang, Chang, Liou, Chen, Chang, Chan et al.: "Peroxisome proliferator-activated receptors ?/mitochondrial uncoupling protein 2 signaling protects against seizure-induced neuronal cell death in the hippocampus following experimental status ..." in: Journal of neuroinflammation, Vol. 9, pp. 184, (2012) (PubMed).

Details zu ENOS

Target: ENOS (NOS3) (Nitric Oxide Synthase 3 (Endothelial Cell) (NOS3))

Andere Bezeichnung: eNOS

Hintergrund:

Synonyms: eNOS, ECNOS, Nitric oxide synthase, endothelial, Constitutive NOS, cNOS, EC-NOS, Endothelial NOS, NOS type III, NOSIII, NOS3

Background: Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets. Isoform eNOS13C: Lacks eNOS activity, dominant-negative form that may down-regulate eNOS activity by forming heterodimers with isoform 1.

Gen-ID: 4846

UniProt: P29474

Pathways: ACE Inhibitor Pathway, Regulation of Systemic Arterial Blood Pressure by Hormones, Cellular Response to Molecule of Bacterial Origin, Myometrial Relaxation and Contraction, Signaling Events mediated by VEGFR1 and VEGFR2, Thromboxane A2 Receptor Signaling, VEGFR1 Specific Signals, VEGF Signaling