TBK1 Antikörper (pSer172)

Produktnummer ABIN746363

Der Kaninchen Polyklonal Anti-TBK1-Antikörper wurde für WB validiert. Er ist geeignet, TBK1 in Proben von Maus, Ratte und Affe zu detektieren. Es sind 3+ Publikationen verfügbar.

Kurzübersicht für TBK1 Antikörper (pSer172) (ABIN746363)

Target: TBK1 (TANK-Binding Kinase 1 (TBK1))

Reaktivität: Maus, Ratte, Affe

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser TBK1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-TBK1 Antikörper

Bindungsspezifität: pSer172

Spezifität: This phosphorylation site is homologous across the listed species.

Kreuzreaktivität: Human, Maus

Aufreinigung: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human TBK1 around the phosphorylation site of Ser172

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Haltbarkeit: 12 months

Referenzen für anti-TBK1 Antikörper (ABIN746363)

Hu, Pan, Cheng, Zhang, Wang, Chen, Zhu, Liu, Yang, Wu, Zhao, Huang, Zhang, Mao, Ou, Yu, Zhang, Liu, Tian, Pan, Rehman, Yin, Jia: "Autophagy Promotes Duck Tembusu Virus Replication by Suppressing p62/SQSTM1-Mediated Innate Immune Responses In Vitro." in: Vaccines, Vol. 8, Issue 1, (2020) (PubMed).

Wan, Chen, Li, Pang, Chen, Nie, Pan, Qiao, Bao: "Porcine FcγRIIb mediated PRRSV ADE infection through inhibiting IFN-β by cytoplasmic inhibitory signal transduction." in: International journal of biological macromolecules, Vol. 138, pp. 198-206, (2020) (PubMed).

Takahashi, Loo, Okada, Kamachi, Watanabe, Wakita, Watanabe, Kawamoto, Miyata, Barber, Ohtani, Hara: "Downregulation of cytoplasmic DNases is implicated in cytoplasmic DNA accumulation and SASP in senescent cells." in: Nature communications, Vol. 9, Issue 1, pp. 1249, (2018) (PubMed).

Details zu TBK1

Target: TBK1 (TANK-Binding Kinase 1 (TBK1))

Andere Bezeichnung: TBK1

Hintergrund:

Synonyms: NAK, T2K, Serine/threonine-protein kinase TBK1, NF-kappa-B-activating kinase, TANK-binding kinase 1, TBK1

Background: Serine/threonine kinase that plays an essential role in regulating inflammatory responses to foreign agents. Following activation of toll-like receptors by viral or bacterial components, associates with TRAF3 and TANK and phosphorylates interferon regulatory factors (IRFs) IRF3 and IRF7 as well as DDX3X. This activity allows subsequent homodimerization and nuclear translocation of the IRFs leading to transcriptional activation of pro-inflammatory and antiviral genes including IFNA and IFNB. In order to establish such an antiviral state, TBK1 form several different complexes whose composition depends on the type of cell and cellular stimuli. Thus, several scaffolding molecules including FADD, TRADD, MAVS, AZI2, TANK or TBKBP1/SINTBAD can be recruited to the TBK1-containing-complexes. Under particular conditions, functions as a NF-kappa-B effector by phosphorylating NF-kappa-B inhibitor alpha/NFKBIA, IKBKB or RELA to translocate NF-Kappa-B to the nucleus. Restricts bacterial proliferation by phosphorylating the autophagy receptor OPTN/Optineurin on 'Ser-177', thus enhancing LC3 binding affinity and antibacterial autophagy. Phosphorylates and activates AKT1. Seems to play a role in energy balance regulation by sustaining a state of chronic, low-grade inflammation in obesity, wich leads to a negative impact on insulin sensitivity. Attenuates retroviral budding by phosphorylating the endosomal sorting complex required for transport-I (ESCRT-I) subunit VPS37C. Phosphorylates Borna disease virus (BDV) P protein.

Gen-ID: 29110

UniProt: Q9UHD2

Pathways: TLR Signalweg, Activation of Innate immune Response, Hepatitis C, Toll-Like Receptors Cascades, SARS-CoV-2 Protein Interaktom