SMAD3 Antikörper (pSer423, pSer425)

Produktnummer ABIN746138

Der Kaninchen Polyklonal Anti-SMAD3-Antikörper wurde für WB, ELISA, IHC (p), FACS, ICC, IF (cc), IF (p) und IHC (fro) validiert. Er ist geeignet, SMAD3 in Proben von Human, Maus, Ratte und Schwein zu detektieren. Es sind 8+ Publikationen verfügbar.

Kurzübersicht für SMAD3 Antikörper (pSer423, pSer425) (ABIN746138)

Target: SMAD3 (SMAD, Mothers Against DPP Homolog 3 (SMAD3))

Reaktivität: Human, Maus, Ratte, Schwein

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser SMAD3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Produktdetails anti-SMAD3 Antikörper

Bindungsspezifität: pSer423, pSer425

Spezifität: These phosphorylation sites are homologous across the species listed.

Kreuzreaktivität: Human, Maus, Schwein, Ratte

Homologie: Dog,Cow,Horse,Chicken

Aufreinigung: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human Smad3 around the phosphorylation site of Ser423/425 [CS(p-S)V(p-S)]

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Haltbarkeit: 12 months

Referenzen für anti-SMAD3 Antikörper (ABIN746138)

Shang, Liu, Liu, Li, Dou, Zhang, Sun, Zhang, Zhu, Mu, Ding, Wen: "Telmisartan improves vascular remodeling through ameliorating prooxidant and profibrotic mechanisms in hypertension via the involvement of transforming growth factor-β1." in: Molecular medicine reports, Vol. 16, Issue 4, pp. 4537-4544, (2018) (PubMed).

Bai, Chu, Wang, Xiang, Yang: "BAMBI promotes porcine granulosa cell steroidogenesis involving TGF-β signaling." in: Theriogenology, Vol. 100, pp. 24-31, (2017) (PubMed).

Zhang, Yang, Li, Xu, Yan, Gao, Jia, Duan: "Angelica polysaccharides inhibit the growth and promote the apoptosis of U251 glioma cells in vitro and in vivo." in: Phytomedicine : international journal of phytotherapy and phytopharmacology, Vol. 33, pp. 21-27, (2017) (PubMed).

Shang, Liu, Liu, Zhang, Mu, Zhu, Liang, Zhai, Ding, Li, Wen: "Acetyl-11-Keto-β-Boswellic Acid Attenuates Prooxidant and Profibrotic Mechanisms Involving Transforming Growth Factor-β1, and Improves Vascular Remodeling in Spontaneously Hypertensive Rats." in: Scientific reports, Vol. 6, pp. 39809, (2016) (PubMed).

Hu, Duan, Li, Wang, Wang, Chu, Sun, Liu, Wang, Lu, Wen: "Hydroxysafflor Yellow A Ameliorates Renal Fibrosis by Suppressing TGF-β1-Induced Epithelial-to-Mesenchymal Transition." in: PLoS ONE, Vol. 11, Issue 4, pp. e0153409, (2016) (PubMed).

Tang, Gao, Mao, He, Liu, Cai, Lin, Wu: "Salidroside protects against bleomycin-induced pulmonary fibrosis: activation of Nrf2-antioxidant signaling, and inhibition of NF-κB and TGF-β1/Smad-2/-3 pathways." in: Cell stress & chaperones, Vol. 21, Issue 2, pp. 239-49, (2016) (PubMed).

Ghiabi, Jiang, Pasquier, Maleki, Abu-Kaoud, Halabi, Guerrouahen, Rafii, Rafii: "Breast cancer cells promote a notch-dependent mesenchymal phenotype in endothelial cells participating to a pro-tumoral niche." in: Journal of translational medicine, Vol. 13, Issue 1, pp. 27, (2015) (PubMed).

Details zu SMAD3

Target: SMAD3 (SMAD, Mothers Against DPP Homolog 3 (SMAD3))

Andere Bezeichnung: Smad3 +

Hintergrund:

Synonyms: LDS3, LDS1C, MADH3, JV15-2, HSPC193, HsT17436, Mothers against decapentaplegic homolog 3, MAD homolog 3, Mad3, Mothers against DPP homolog 3, hMAD-3, SMAD family member 3, SMAD 3, Smad3, hSMAD3

Background: Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD3/SMAD4 complex, activates transcription. Also can form a SMAD3/SMAD4/JUN/FOS complex at the AP-1/SMAD site to regulate TGF-beta-mediated transcription. Has an inhibitory effect on wound healing probably by modulating both growth and migration of primary keratinocytes and by altering the TGF-mediated chemotaxis of monocytes. This effect on wound healing appears to be hormone-sensitive. Regulator of chondrogenesis and osteogenesis and inhibits early healing of bone fractures. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator.

Gen-ID: 4088

UniProt: P84022

Pathways: Zellzyklus, Chromatin Binding, Cell-Cell Junction Organization, Positive Regulation of Endopeptidase Activity, Autophagie