RAPTOR Antikörper (pSer792)

Produktnummer ABIN745478

Der Kaninchen Polyklonal Anti-RAPTOR-Antikörper wurde für ELISA, IF (cc), IF (p), IHC (p) und IHC (fro) validiert. Er ist geeignet, RAPTOR in Proben von Human, Maus und Zebrafisch (Danio rerio) zu detektieren.

Kurzübersicht für RAPTOR Antikörper (pSer792) (ABIN745478)

Target: RAPTOR

Reaktivität: Human, Maus, Zebrafisch (Danio rerio)

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser RAPTOR Antikörper ist unkonjugiert

Applikation: ELISA, Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Produktdetails anti-RAPTOR Antikörper

Bindungsspezifität: pSer792

Kreuzreaktivität: Human, Maus, Zebrafisch (Danio rerio)

Homologie: Rat,Dog,Cow,Sheep,Pig,Horse,Chicken,Guinea Pig

Aufreinigung: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from mouse Raptor around the phosphorylation site of Ser792

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Haltbarkeit: 12 months

Details zu RAPTOR

Target: RAPTOR

Andere Bezeichnung: Raptor

Hintergrund:

Synonyms: ARaptor phospho S792, Raptor phospho Ser792, p-Raptor Ser792, p150 target of rapamycin TOR scaffold protein containing WD repeats, P150 target of rapamycin TOR-scaffold protein, Regulatory Associated Protein of mTOR, KIAA1303, RPTOR_HUMAN, Regulatory-associated protein of mTOR, p150 target of rapamycin TOR-scaffold protein.

Background: mTOR controls cell growth, in part by regulating p70 S6 kinase alpha (p70alpha) and eukaryotic initiation factor 4E binding protein 1 (4EBP1). Raptor is a 150 kDa mTOR binding protein that also binds 4EBP1 and p70alpha. The binding of Raptor to mTOR is necessary for the mTOR-catalyzed phosphorylation of 4EBP1 in vitro, and it strongly enhances the mTOR kinase activity toward p70alpha. Rapamycin or amino acid withdrawal increases, whereas insulin strongly inhibits, the recovery of 4EBP1 and raptor on 7-methyl-GTP Sepharose. Partial inhibition of raptor expression by RNA interference (RNAi) reduces mTOR-catalyzed 4EBP1 phosphorylation in vitro. RNAi of C. elegans raptor yields an array of phenotypes that closely resemble those produced by inactivation of Ce-TOR. Thus, raptor is an essential scaffold for the mTOR-catalyzed phosphorylation of 4EBP1 and mediates TOR action in vivo.

Pathways: Warburg Effekt