EPH Receptor B1 Antikörper (Extracellular, N-Term)

Produktnummer ABIN7884940

Der Kaninchen Polyklonal anti-EPH Receptor B1 Antikörper wird verwendet zum Nachweis von EPH Receptor B1 in Proben von Human, Maus und Ratte. Er wurde validiert für WB, IF, FACS, IC und LCI.

Kurzübersicht für EPH Receptor B1 Antikörper (Extracellular, N-Term) (ABIN7884940)

Target: EPH Receptor B1 (EPHB1)

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser EPH Receptor B1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunochromatography (IC), Live Cell Imaging (LCI)

Güteklasse: Carrier-free

Produktdetails anti-EPH Receptor B1 Antikörper

Bindungsspezifität: AA 485-497, Extracellular, N-Term

Verwendungszweck: A Rabbit Polyclonal Antibody to EphB1 Receptor

Homologie: Rat,human - identical

Aufreinigung: Affinity purified on immobilized antigen.

Immunogen: (C)RSQTNTARIDGLR, corresponding to amino acid residues 485-497 of mouse EphB1

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

WB: 1:800

FC: 1:15

ICC: 1:100

IHC: The optimal concentration should be determined by the user

IP: The optimal concentration should be determined by the user

Kommentare:

Negative Control: (ABIN7235294)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: 0.2 mL double distilled water (DDW)

Konzentration: 1 mg/mL

Buffer: PBS pH 7.4

Konservierungsmittel: Without preservative

Lagerung: -20 °C

Informationen zur Lagerung: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Details zu EPH Receptor B1

Target: EPH Receptor B1 (EPHB1)

Andere Bezeichnung: Ephrin type-B receptor 1

Hintergrund:

Synonyms: Ephrin type-B receptor 1, Eph receptor B1, Tyrosine-protein kinase receptor EPH-2

Description: Eph receptors are the largest family of receptor tyrosine kinases (RTKs). EphA receptors (EphA1-10) bind ephrinA ligands which are GPI-linked proteins and EphB receptors (EphB1-6) bind ephrinB ligands which are membrane protein with one transmembrane domain1,2. Within each subfamily, interactions between receptor and ligand are promiscuous. In addition, Eph receptors can also bind ephrins from the other class2. Forward and reverse signaling through Eph receptors is a unique characteristic to this RTK since ephrins are physically linked to the plasma membrane3.Structurally, Eph receptors contain an extracellular ligand-binding domain, a transmembrane domain and an intracellular C-terminal domain responsible for intracellular signaling1. Forward Eph receptor signaling involves autophosphorylation of the receptor via a tyrosine kinase domain, as well as phosphorylation of other proteins. Known effectors of the forward signaling include Src kinase and Ras/Rho GTPases2. Much less is known about the reverse signaling mediated by Eph receptors. Besides from acting independently, Eph receptors can also signal in concert with other receptors. For example, Eph receptors cooperate with FGF receptor, NMDA ligand-gated ion channel and chemokine G-protein coupled receptor2.Biological activities attributed to the Eph receptor-ephrin signaling module include establishing neuronal connections, mediating neuronal plasticity and repair following neuronal injury2,4. Eph receptors may also have a role in the immune system5.Eph receptors are expressed in the developing nervous system, and in the adult brain. It is also detected in the pancreas, intestine, bone and lymphocytes. In cancer cells, Eph receptors and ephrins are overexpressed2,6,7. They are also implicated in neurodegenerative disorders like Alzheimer's disease1.

Gen-ID: 270190

UniProt: Q8CBF3

Pathways: RTK Signalweg