Rekombinanter PSMC5 Antikörper

Produktnummer ABIN7882067

Der Kaninchen Monoklonal anti-PSMC5 Antikörper (Klon 29P84) (ABIN7882067) detektiert spezifisch PSMC5 in WB, IF, IHC, IP, ICC und FACS. Dieser Antikörper reagiert spezifisch mit Proben aus Human, Maus und Ratte.

Kurzübersicht für Rekombinanter PSMC5 Antikörper (ABIN7882067)

Target: PSMC5 (Proteasome (Prosome, Macropain) 26S Subunit, ATPase, 5 (PSMC5))

Antikörpertyp: Recombinant Antibody

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Monoklonal

Konjugat: Dieser PSMC5 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Klon: 29P84

Produktdetails anti-PSMC5 Antikörper

Verwendungszweck: PSMC5 Antibody / 26S proteasome regulatory subunit 8

Aufreinigung: Affinity chromatography

Immunogen: A synthesized peptide derived from human PSMC5 was used as the immunogen for the PSMC5 antibody.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the PSMC5 antibody should be determined by the researcher.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol, 0.4-0.5 mg/mL BSA

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store the PSMC5 antibody at -20oC.

Details zu PSMC5

Target: PSMC5 (Proteasome (Prosome, Macropain) 26S Subunit, ATPase, 5 (PSMC5))

Andere Bezeichnung: PSMC5

Hintergrund: PSMC5 antibody detects 26S proteasome regulatory subunit 8, encoded by the PSMC5 gene. This protein is one of the six ATPases of the AAA family that form the base of the 19S regulatory particle of the proteasome. PSMC5, also known as TBP1, provides ATP hydrolysis required for unfolding and translocating ubiquitinated substrates into the 20S core particle for degradation. PSMC5 antibody provides researchers with a reliable tool to study protein homeostasis, cell cycle regulation, and signaling pathways dependent on proteasomal degradation.

The 26S proteasome is responsible for the majority of regulated intracellular protein degradation in eukaryotic cells. By removing misfolded proteins, damaged proteins, and short lived regulatory proteins, the proteasome maintains proteostasis and controls key signaling events. Research using PSMC5 antibody has shown that 26S proteasome regulatory subunit 8 contributes to ATP driven unfolding, ensuring substrate entry into the proteolytic chamber. This function is essential for turnover of cell cycle regulators, transcription factors, and signal transduction intermediates.

Dysfunction of the proteasome contributes to numerous diseases, including neurodegenerative disorders such as Parkinson and Alzheimer disease, where accumulation of protein aggregates is a hallmark. Research with PSMC5 antibody has demonstrated that altered proteasome activity leads to impaired clearance of aggregation prone proteins. In cancer, hyperactivation of the proteasome allows degradation of tumor suppressors and cell cycle inhibitors, promoting unchecked proliferation. PSMC5 has also been implicated in immune regulation, where proteasomal processing generates antigenic peptides presented on MHC class I molecules.

PSMC5 antibody is widely used in western blotting, immunoprecipitation, and immunohistochemistry. Western blotting demonstrates abundance in metabolically active tissues, while immunoprecipitation allows isolation of 19S regulatory complexes containing PSMC5. Immunohistochemistry highlights proteasome distribution in brain, muscle, and immune organs. These applications make PSMC5 antibody versatile in both fundamental and translational research.

By supplying validated PSMC5 antibody reagents, NSJ Bioreagents supports research into proteasome biology, neurodegeneration, and oncology. Detection of 26S proteasome regulatory subunit 8 ensures that researchers can examine how protein degradation maintains cellular health and contributes to disease when dysregulated.

UniProt: P62195

Pathways: Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA, Ubiquitin Proteasome Pathway