Rekombinanter PSIP1 Antikörper

Produktnummer ABIN7881909

Dieser Kaninchen Monoklonal Antikörper detektiert spezifisch PSIP1 in WB, IHC, ICC und IF. Es zeigt Reaktivität gegenüber Proben von Human.

Kurzübersicht für Rekombinanter PSIP1 Antikörper (ABIN7881909)

Target: PSIP1 (PC4 and SFRS1 Interacting Protein 1 (PSIP1))

Antikörpertyp: Recombinant Antibody

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Monoklonal

Konjugat: Dieser PSIP1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF)

Klon: 31P66

Produktdetails anti-PSIP1 Antikörper

Verwendungszweck: PSIP1 Antibody / PC4 and SFRS1-interacting protein / LEDGF

Aufreinigung: Affinity chromatography

Immunogen: A synthesized peptide derived from human PSIP1 / LEDGF was used as the immunogen for the PSIP1 antibody.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the PSIP1 antibody should be determined by the researcher.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol, 0.4-0.5 mg/mL BSA

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store the PSIP1 antibody at -20oC.

Details zu PSIP1

Target: PSIP1 (PC4 and SFRS1 Interacting Protein 1 (PSIP1))

Andere Bezeichnung: PSIP1

Hintergrund: PSIP1 antibody detects Lens epithelium derived growth factor, encoded by the PSIP1 gene. Lens epithelium derived growth factor, also known as LEDGF, is a transcription coactivator that plays important roles in stress response, survival signaling, and gene regulation. It binds chromatin through its PWWP domain and recruits transcriptional machinery to stress inducible genes, helping cells adapt to oxidative and environmental stress. PSIP1 antibody provides a means to study this protein's functions in transcriptional control, survival pathways, and viral integration.

Lens epithelium derived growth factor is best known for its role in HIV infection, where it acts as a tethering factor for integrase, directing viral DNA integration into active chromatin regions. Research using PSIP1 antibody has confirmed that depletion of LEDGF reduces viral replication efficiency, underscoring its importance in retroviral life cycles. Beyond virology, PSIP1 regulates stress response genes, including those activated by heat shock and oxidative stress, contributing to cellular resilience. By detecting this protein with PSIP1 antibody, researchers gain insight into how cells survive hostile environments.

PSIP1 also influences development and cancer. Overexpression of LEDGF has been observed in leukemia and solid tumors, where it enhances survival pathways and confers resistance to apoptosis. Chromosomal translocations involving PSIP1 result in fusion proteins that act as oncogenic drivers, particularly in mixed lineage leukemia. Studies employing PSIP1 antibody have demonstrated altered transcriptional programs and stress resistance in tumor cells, linking LEDGF to oncogenesis and therapeutic resistance. Because of these functions, PSIP1 remains a focus in drug discovery and biomarker research.

PSIP1 antibody is applied in western blotting, chromatin immunoprecipitation, and immunohistochemistry. Western blotting demonstrates isoform expression and stress inducible regulation. Chromatin immunoprecipitation with PSIP1 antibody identifies genomic targets of LEDGF, revealing pathways controlled by this coactivator. Immunohistochemistry highlights tissue distribution, while functional assays demonstrate its ability to modulate transcriptional activity under stress. These diverse applications underscore the importance of PSIP1 antibody in molecular and cellular research.

By supplying validated PSIP1 antibody reagents, NSJ Bioreagents supports investigations into transcriptional regulation, viral biology, and cancer progression. Detection of Lens epithelium derived growth factor provides a reliable tool for understanding how this protein integrates stress response pathways with gene regulation and disease.

UniProt: O75475

Pathways: Chromatin Binding, Ribonucleoprotein Complex Subunit Organization