MGMT Antikörper

Produktnummer ABIN7881875

Der Maus Monoklonal anti-MGMT Antikörper (Klon MGMT-4791) (ABIN7881875) detektiert spezifisch MGMT in IHC (p). Dieser Antikörper reagiert spezifisch mit Proben aus Human.

Kurzübersicht für MGMT Antikörper (ABIN7881875)

Target: MGMT (O6-Methylguanine-DNA-Methyltransferase (MGMT))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser MGMT Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Klon: MGMT-4791

Produktdetails anti-MGMT Antikörper

Verwendungszweck: MGMT Antibody

Aufreinigung: Protein A/G affinity

Immunogen: Purified His-tagged MGMT protein was used as the immunogen for the MGMT antibody.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the MGMT antibody should be determined by the researcher.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.2 mg/mL

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot the MGMT antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Details zu MGMT

Target: MGMT (O6-Methylguanine-DNA-Methyltransferase (MGMT))

Andere Bezeichnung: MGMT

Hintergrund: Cancer chemotherapeutic alkylating agents (e.g. BCNU,) act by inducing formation of lethal cross links at the O6 alkylguanine position in DNA. MGMT transfers alkyl adducts from the O6 position of guanine in DNA (prior to cross link formation) to a cysteine residue in its own sequence, thereby restoring DNA to its intact state. This transfer inactivates the MGMT enzyme and is irreversible, hence the level of MGMT in a cell is directly proportional to the level of DNA damage it can tolerate. In normal tissues, MGMT acts as a suppressor of mutation and carcinogenesis. Tumors with high levels of MGMT are likely to be drug resistant

UniProt: P16455

Pathways: DNA Reparatur, Positive Regulation of Response to DNA Damage Stimulus