Rekombinanter DOT1L Antikörper

Produktnummer ABIN7880643

Der Kaninchen Monoklonal anti-DOT1L Antikörper wird verwendet zum Nachweis von DOT1L in Proben von Human und Maus. Er wurde validiert für WB und IHC (p).

Kurzübersicht für Rekombinanter DOT1L Antikörper (ABIN7880643)

Target: DOT1L (DOT1-Like, Histone H3 Methyltransferase (S. Cerevisiae) (DOT1L))

Antikörpertyp: Recombinant Antibody

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Monoklonal

Konjugat: Dieser DOT1L Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Klon: CBF-4

Produktdetails anti-DOT1L Antikörper

Verwendungszweck: DOT1L Antibody / KMT4

Aufreinigung: Affinity purified

Immunogen: A synthetic peptide specific to Disruptor of telomeric silencing 1 like protein was used as the immunogen for the DOT1L antibody.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the DOT1L antibody should be determined by the researcher.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Antibody in PBS with 0.02 % sodium azide, 50 % glycerol and 0.4-0.5 mg/mL BSA

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store the DOT1L antibody at -20oC.

Details zu DOT1L

Target: DOT1L (DOT1-Like, Histone H3 Methyltransferase (S. Cerevisiae) (DOT1L))

Andere Bezeichnung: DOT1L

Hintergrund: DOT1L antibody is a widely used reagent for studying epigenetic control, chromatin remodeling, and transcriptional regulation. The encoded protein, KMT4 (also known as disruptor of telomeric silencing 1 like), is a histone methyltransferase that specifically methylates histone H3 on lysine 79. This unique modification, located within the globular domain of histone H3, is strongly linked to transcriptional activation, DNA repair, and maintenance of genomic stability. Unlike most lysine methyltransferases that contain a SET domain, DOT1L is structurally distinct, highlighting its specialized role in epigenetic regulation.

KMT4 functions by altering chromatin accessibility and creating binding sites for regulatory proteins that control transcription and DNA damage response. During development, DOT1L plays critical roles in embryogenesis, hematopoiesis, and differentiation. Loss of DOT1L disrupts stem cell renewal and lineage commitment, demonstrating its essential role in developmental biology. Its activity is also important for maintaining telomeric silencing and ensuring proper chromosomal function.

Aberrant DOT1L activity has been strongly implicated in cancer. In mixed lineage leukemia (MLL) rearranged leukemias, DOT1L is aberrantly recruited to oncogenic transcriptional complexes, leading to inappropriate H3K79 methylation and activation of leukemia promoting genes. This discovery has made DOT1L a therapeutic target, and small molecule inhibitors of DOT1L are in clinical trials for treatment of MLL associated leukemias. Beyond leukemia, dysregulated DOT1L activity has been observed in solid tumors, cardiovascular disease, and viral infection, underscoring its wide biological significance.

At the molecular level, DOT1L associates with transcriptional elongation complexes and other chromatin modifiers, linking histone modification with gene expression control. Structural studies show that DOT1L interacts directly with the nucleosome core, uniquely positioning it to catalyze methylation of H3K79. Through these mechanisms, DOT1L serves as an integrator of epigenetic signaling and transcriptional activity, shaping the cellular transcriptome in response to developmental and environmental signals.

The DOT1L antibody is commonly used in western blotting, immunohistochemistry, immunofluorescence, and flow cytometry to detect expression levels, nuclear localization, and disease associated changes. These applications are valuable for exploring transcriptional regulation, epigenetic therapy, and cancer biology. For researchers studying chromatin structure, RNA polymerase II elongation, or targeted therapeutics, the DOT1L antibody provides a reliable and specific detection tool. NSJ Bioreagents offers validated antibodies designed to ensure accuracy and reproducibility in advanced molecular research.

UniProt: Q8TEK3