DOT1L Antikörper (C-Term)

Produktnummer ABIN6971602

Der Kaninchen Polyklonal anti-DOT1L Antikörper (ABIN6971602) detektiert spezifisch DOT1L in WB. Dieser Antikörper reagiert spezifisch mit Proben aus Human.

Kurzübersicht für DOT1L Antikörper (C-Term) (ABIN6971602)

Target: DOT1L (DOT1-Like, Histone H3 Methyltransferase (S. Cerevisiae) (DOT1L))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser DOT1L Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-DOT1L Antikörper

Bindungsspezifität: C-Term

Produktmerkmale: DOT1L (Disruptor of Telomeric silencing 1-like, also known as KMT4) is the mammalian homologue of the yeast Dot1 protein and the Drosophila Grappa protein. DOTL1 is the enzyme responsible for lysine 79 methylation of histone H3. DOT1L can promote an oncogenic pattern of gene expression through binding with several MLL fusion partners found in acute leukemia, thus leading to aberrant methylation of lysine 79 at MLL target genes. In contrast to most other histone lysine methyltransferases, DOT1L does not contain a SET domain. Biochemical studies suggest that recombinant DOT1L is active on recombinant nucleosomes. DOT1L antibody (pAb) was raised in a Rabbit host. It has been validated for use in Western blot, it has been shown to react with Human samples.

Aufreinigung: Affinity Purified

Immunogen: This DOT1L antibody was raised against a peptide derived from the C-terminal region of human DOT1L.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: Purified IgG in 70 mM Tris ( pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30 % glycerol and 0.035 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Details zu DOT1L

Target: DOT1L (DOT1-Like, Histone H3 Methyltransferase (S. Cerevisiae) (DOT1L))

Andere Bezeichnung: DOT1L

Molekulargewicht: 200 kDa

NCBI Accession: NP_115871