ATG4A Antikörper

Produktnummer ABIN7880031

Der Maus Monoklonal anti-ATG4A Antikörper wird verwendet zum Nachweis von ATG4A in Proben von Human. Er wurde validiert für WB, IF, IHC (p) und FACS.

Kurzübersicht für ATG4A Antikörper (ABIN7880031)

Target: ATG4A (Autophagy related 4A Cysteine Peptidase (ATG4A))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser ATG4A Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS)

Klon: 1458CT808-66-25-69

Produktdetails anti-ATG4A Antikörper

Verwendungszweck: ATG4A Antibody

Aufreinigung: Protein G affinity

Immunogen: Recombinant human protein was used as the immunogen for the ATG4A antibody.

Isotyp: IgG2b, kappa

Anwendungsinformationen

Applikationshinweise: The stated application concentrations are suggested starting points. Titration of the ATG4A antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: In 1X PBS, pH 7.4, with 0.09 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Aliquot the ATG4A antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Details zu ATG4A

Target: ATG4A (Autophagy related 4A Cysteine Peptidase (ATG4A))

Andere Bezeichnung: ATG4A

Hintergrund: Cysteine protease required for the cytoplasm to vacuole transport (Cvt) and autophagy. Cleaves the C-terminal amino acid of ATG8 family proteins to reveal a C-terminal glycine. Exposure of the glycine at the C-terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, which is necessary for autophagy. Preferred substrate is GABARAPL2 followed by MAP1LC3A and GABARAP. Has also an activity of delipidating enzyme for the PE-conjugated forms.

UniProt: Q8WYN0

Pathways: Autophagie