ATG4A Antikörper (AA 1-30)

Produktnummer ABIN388491

Der Kaninchen Polyklonal anti-ATG4A Antikörper (Klon RB7493) (ABIN388491) detektiert spezifisch ATG4A in WB und IHC (p). Dieser Antikörper reagiert spezifisch mit Proben aus Human und Maus.

Kurzübersicht für ATG4A Antikörper (AA 1-30) (ABIN388491)

Target: ATG4A (Autophagy related 4A Cysteine Peptidase (ATG4A))

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser ATG4A Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Klon: RB7493

Produktdetails anti-ATG4A Antikörper

Bindungsspezifität: AA 1-30

Aufreinigung: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This ATG4A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from human ATG4A.

Isotyp: Ig Fraction

Anwendungsinformationen

Applikationshinweise: WB: 1:1000. IHC-P: 1:50~100

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Haltbarkeit: 6 months

Details zu ATG4A

Target: ATG4A (Autophagy related 4A Cysteine Peptidase (ATG4A))

Andere Bezeichnung: ATG4A

Hintergrund: Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). APG4A is a cysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes. Preferred substrate is GABARAPL2 followed by MAP1LC3A and GABARAP.

Molekulargewicht: 45378

Gen-ID: 115201

NCBI Accession: NP_443168, NP_840054

UniProt: Q8WYN0

Pathways: Autophagie