MLH1 Antikörper (full length)

Produktnummer ABIN7878675

Der Maus Monoklonal anti-MLH1 Antikörper wird verwendet zum Nachweis von MLH1 in Proben von Human. Er wurde validiert für IHC (p).

Kurzübersicht für MLH1 Antikörper (full length) (ABIN7878675)

Target: MLH1 (MutL Homolog 1 (MLH1))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser MLH1 Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Güteklasse: Carrier-free

Klon: MLH1-7563

Produktdetails anti-MLH1 Antikörper

Bindungsspezifität: full length

Verwendungszweck: MutL Homolog 1 Antibody / MLH1 (azide and preservative free)

Aufreinigung: Protein A/G affinity

Immunogen: Recombinant full-length human MLH1 protein was used as the immunogen for the MutL Homolog 1 antibody.

Isotyp: IgG1, kappa

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the MutL Homolog 1 antibody should be determined by the researcher.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Konservierungsmittel: Azide free

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot the MutL Homolog 1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Details zu MLH1

Target: MLH1 (MutL Homolog 1 (MLH1))

Andere Bezeichnung: MutL Homolog 1

Hintergrund: This mAb recognizes a protein of 83 kDa, identified as MLH1. Defects in MLH1 are the cause of hereditary non-polyposis colorectal cancer type 2 (HNPCC2).Heterodimerizes with PMS2 to form MutL alpha, a component of the post-replicative DNA mismatch repair system (MMR). DNA repair is initiated by MutS alpha (MSH2-MSH6) or MutS beta (MSH2-MSH6) binding to a dsDNA mismatch, then MutL alpha is recruited to the heteroduplex. Assembly of the MutL-MutS-heteroduplex ternary complex in presence of RFC and PCNA is sufficient to activate endonuclease activity of PMS2. It introduces single-strand breaks near the mismatch and thus generates new entry points for the exonuclease EXO1 to degrade the strand containing the mismatch. DNA methylation would prevent cleavage and therefore assure that only the newly mutated DNA strand is going to be corrected. MutL alpha (MLH1-PMS2) interacts physically with the clamp loader subunits of DNA polymerase III, suggesting that it may play a role to recruit the DNA polymerase III to the site of the MMR. Also implicated in DNA damage signaling, a process, which induces cell cycle arrest and can lead to apoptosis in case of major DNA damages. Heterodimerizes with MLH3 to form MutL gamma, which plays a role in meiosis.

UniProt: P40692

Pathways: DNA Reparatur, Production of Molecular Mediator of Immune Response