PIK3C3 Antikörper (C-Term)

Produktnummer ABIN7877455

Der Kaninchen Polyklonal anti-PIK3C3 Antikörper (ABIN7877455) detektiert spezifisch PIK3C3 in WB und FACS. Dieser Antikörper reagiert spezifisch mit Proben aus Human, Maus und Ratte.

Kurzübersicht für PIK3C3 Antikörper (C-Term) (ABIN7877455)

Target: PIK3C3 (Phosphoinositide-3-Kinase, Class 3 (PIK3C3))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PIK3C3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Flow Cytometry (FACS)

Produktdetails anti-PIK3C3 Antikörper

Bindungsspezifität: C-Term

Verwendungszweck: PIK3C3 Antibody / VPS34 / Phosphatidylinositol 3-kinase catalytic subunit type 3

Aufreinigung: Immunogen affinity purified

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human VPS34/PIK3C3 was used as the immunogen for the PIK3C3 antibody.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the PIK3C3 antibody should be determined by the researcher.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: After reconstitution, the PIK3C3 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Details zu PIK3C3

Target: PIK3C3 (Phosphoinositide-3-Kinase, Class 3 (PIK3C3))

Andere Bezeichnung: PIK3C3

Hintergrund: PIK3C3 antibody detects Phosphatidylinositol 3-kinase catalytic subunit type 3, a lipid kinase that generates phosphatidylinositol 3-phosphate (PI3P) to control autophagy and endosomal trafficking. The UniProt recommended name is Phosphatidylinositol 3-kinase catalytic subunit type 3 (PIK3C3), also known as Vacuolar protein sorting-associated protein 34 (VPS34). This enzyme is the only class III PI3K in mammalian cells and plays a pivotal role in autophagosome formation and lysosomal function.

Functionally, PIK3C3 antibody recognizes a 887-amino-acid cytoplasmic protein that catalyzes the phosphorylation of phosphatidylinositol to generate PI3P, recruiting downstream effectors such as WIPI and DFCP1. PIK3C3 forms multiprotein complexes with Beclin 1, UVRAG, ATG14, and VPS15 to regulate membrane nucleation during autophagy and endosome maturation. Its lipid kinase activity is crucial for endocytic trafficking, nutrient sensing, and organelle biogenesis.

The PIK3C3 gene, located on chromosome 18q12.3, is ubiquitously expressed and evolutionarily conserved from yeast to humans. It governs autophagic flux by mediating vesicle formation and fusion with lysosomes. Through its interaction with Beclin 1, PIK3C3 serves as a core regulator of cellular homeostasis under nutrient stress and is involved in tumor suppression and neuroprotection.

Pathologically, dysregulation of PIK3C3/VPS34 contributes to cancer, neurodegeneration, and infectious disease. Inhibition of VPS34 impairs autophagy, leading to protein aggregation and metabolic imbalance. Conversely, enhanced PIK3C3 activity supports tumor cell survival under nutrient deprivation. The enzyme is also hijacked by viruses to generate replication compartments. Research using PIK3C3 antibody advances understanding of autophagic control, endosomal transport, and signaling adaptation to stress.

PIK3C3 antibody is suitable for western blotting, immunofluorescence, and immunoprecipitation to detect VPS34 and its complexes. It supports studies in autophagy, intracellular trafficking, and lipid signaling. NSJ Bioreagents provides validated PIK3C3 antibody reagents optimized for autophagy and vesicle biogenesis research.

Structurally, PIK3C3 consists of a helical domain, a C2 domain for membrane association, and a lipid kinase catalytic domain. Its activity depends on interactions with VPS15 and Beclin 1 for stabilization and subcellular localization. This antibody facilitates analysis of PIK3C3/VPS34's central role in autophagy initiation and endolysosomal trafficking.

UniProt: Q8NEB9

Pathways: AMPK Signaling, Activation of Innate immune Response, Inositol Metabolic Process, Toll-Like Receptors Cascades, Autophagie