MYL3/CMLC1 Antikörper (C-Term)

Produktnummer ABIN7877434

Der Kaninchen Polyklonal anti-MYL3/CMLC1 Antikörper (ABIN7877434) detektiert spezifisch MYL3/CMLC1 in WB. Dieser Antikörper reagiert spezifisch mit Proben aus Human, Maus und Ratte.

Kurzübersicht für MYL3/CMLC1 Antikörper (C-Term) (ABIN7877434)

Target: MYL3/CMLC1 (MYL3) (Myosin, Light Chain 3 (MYL3))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser MYL3/CMLC1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-MYL3/CMLC1 Antikörper

Bindungsspezifität: C-Term

Verwendungszweck: MYL3 Antibody / Myosin light chain 3

Aufreinigung: Immunogen affinity purified

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human MYL3 was used as the immunogen for the MYL3 antibody.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the MYL3 antibody should be determined by the researcher.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: After reconstitution, the MYL3 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Details zu MYL3/CMLC1

Target: MYL3/CMLC1 (MYL3) (Myosin, Light Chain 3 (MYL3))

Andere Bezeichnung: MYL3

Hintergrund: MYL3 antibody detects Myosin light chain 3, a contractile protein component of cardiac and skeletal muscle that modulates actin-myosin interactions during muscle contraction. The UniProt recommended name is Myosin light chain 3 (MYL3). This protein binds to myosin heavy chains and contributes to force generation and sarcomere stability in striated muscle fibers.

Functionally, MYL3 antibody identifies a 151-amino-acid calcium-binding protein localized to the myofilament's thick filament region. MYL3 interacts with the myosin heavy chain alpha isoform and regulates actomyosin ATPase activity through conformational changes induced by calcium binding. It plays a key role in sarcomere assembly, contractile force regulation, and muscle fiber maintenance.

The MYL3 gene is located on chromosome 3p21.31 and is expressed predominantly in ventricular cardiac tissue and slow-twitch skeletal muscles. MYL3 is essential for normal heart contractility and mechanical stability under physiological stress.

Pathologically, mutations in MYL3 cause familial hypertrophic cardiomyopathy and restrictive cardiomyopathy by altering calcium binding and myosin-actin interactions. Defects in MYL3 impair contractile performance and promote myofibrillar disarray. Research using MYL3 antibody supports studies in cardiac physiology, muscle biochemistry, and hereditary myopathies.

MYL3 antibody is validated for western blotting, immunohistochemistry, and immunofluorescence to detect sarcomeric proteins. NSJ Bioreagents provides MYL3 antibody reagents optimized for research in cardiomyocyte biology, muscle contraction, and structural myopathy studies.

Structurally, Myosin light chain 3 belongs to the EF-hand superfamily and contains two calcium-binding motifs that regulate its conformational changes and interaction with myosin. This antibody aids analysis of MYL3's role in cardiac contractility and muscle development.

UniProt: P08590