TXNRD1 Antikörper (AA 93-326)

Produktnummer ABIN7877180

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch TXNRD1 in WB und ELISA. Es zeigt Reaktivität gegenüber Proben von Human.

Kurzübersicht für TXNRD1 Antikörper (AA 93-326) (ABIN7877180)

Target: TXNRD1 (Thioredoxin Reductase 1 (TXNRD1))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser TXNRD1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Produktdetails anti-TXNRD1 Antikörper

Bindungsspezifität: AA 93-326

Verwendungszweck: TXNRD1 Antibody / Thioredoxin reductase 1

Aufreinigung: Immunogen affinity purified

Immunogen: E.coli-derived human TXNRD1 recombinant protein (Position: Y93-Y326) was used as the immunogen for the TXNRD1 antibody.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the TXNRD1 antibody should be determined by the researcher.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: After reconstitution, the TXNRD1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Details zu TXNRD1

Target: TXNRD1 (Thioredoxin Reductase 1 (TXNRD1))

Andere Bezeichnung: TXNRD1

Hintergrund: TXNRD1 antibody detects Thioredoxin reductase 1, a selenocysteine-containing enzyme that maintains cellular redox balance by catalyzing the NADPH-dependent reduction of thioredoxin. The UniProt recommended name is Thioredoxin reductase 1 (TXNRD1). This enzyme is a central component of the thioredoxin system, which regulates antioxidant defense, DNA synthesis, and redox signaling.

Functionally, TXNRD1 antibody identifies a 654-amino-acid cytosolic enzyme composed of an FAD-binding domain, NADPH-binding domain, and a selenocysteine-containing active site motif (GCUG). TXNRD1 reduces oxidized thioredoxin, which in turn regenerates reduced cysteine residues in target proteins, maintaining redox homeostasis. It also directly reduces lipid hydroperoxides, hydrogen peroxide, and other disulfide-containing molecules, contributing to cellular antioxidant capacity.

The TXNRD1 gene is located on chromosome 12q23.3 and encodes the cytosolic isoform of thioredoxin reductase. TXNRD1 expression is regulated by the transcription factor NRF2 through the antioxidant response element (ARE) under oxidative or electrophilic stress. Its activity is essential for maintaining a reducing intracellular environment, supporting cell survival, and modulating signaling pathways such as apoptosis and proliferation.

In redox biology, TXNRD1 plays a dual role: it protects cells from oxidative stress yet also supports cancer cell survival under high metabolic demand. Elevated TXNRD1 expression is frequently observed in tumors, where it promotes resistance to chemotherapeutic agents and radiotherapy. Conversely, TXNRD1 inhibition induces oxidative stress, mitochondrial dysfunction, and apoptosis, making it a therapeutic target for cancer and inflammatory diseases.

Beyond oncology, TXNRD1 contributes to immune function, lipid metabolism, and cardiovascular health. Its deficiency leads to increased susceptibility to oxidative damage, accelerated aging, and metabolic imbalance. In experimental models, altered TXNRD1 expression affects insulin sensitivity and lipid peroxidation levels, linking it to metabolic and neurodegenerative disorders.

TXNRD1 antibody is widely used in redox biology, metabolism, and cancer research. It is suitable for western blotting, immunohistochemistry, and enzymatic assays to monitor TXNRD1 expression and activity. This antibody supports investigations into antioxidant defense, redox signaling, and selenoprotein regulation. In pharmacology, it helps evaluate TXNRD1 as a biomarker of oxidative stress and drug response.

Structurally, TXNRD1 forms a homodimer with active sites on each subunit and utilizes FAD and NADPH cofactors for electron transfer. Its C-terminal selenocysteine residue is critical for catalytic turnover. NSJ Bioreagents provides TXNRD1 antibody reagents validated for use in redox homeostasis, antioxidant enzyme, and cancer metabolism research.

UniProt: Q16881

Pathways: Regulation of Lipid Metabolism by PPARalpha, Regulation of Carbohydrate Metabolic Process, Cell RedoxHomeostasis