CDCA8 Antikörper (AA 72-280)

Produktnummer ABIN7876633

Der Kaninchen Polyklonal anti-CDCA8 Antikörper (ABIN7876633) detektiert spezifisch CDCA8 in WB, ELISA, FACS, IF und ICC. Dieser Antikörper reagiert spezifisch mit Proben aus Human.

Kurzübersicht für CDCA8 Antikörper (AA 72-280) (ABIN7876633)

Target: CDCA8 (Cell Division Cycle Associated 8 (CDCA8))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CDCA8 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunofluorescence (IF), Immunocytochemistry (ICC)

Produktdetails anti-CDCA8 Antikörper

Bindungsspezifität: AA 72-280

Verwendungszweck: CDCA8 Antibody / Cell division cycle-associated protein 8 / Borealin

Aufreinigung: Immunogen affinity purified

Immunogen: E.coli-derived human CDCA8 recombinant protein (Position: D72-K280) was used as the immunogen for the CDCA8 antibody.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the CDCA8 antibody should be determined by the researcher.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: After reconstitution, the CDCA8 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Details zu CDCA8

Target: CDCA8 (Cell Division Cycle Associated 8 (CDCA8))

Andere Bezeichnung: CDCA8

Hintergrund: CDCA8 antibody recognizes Cell division cycle-associated protein 8, also known as Borealin, a core component of the chromosomal passenger complex (CPC) that governs chromosome segregation, spindle assembly, and cytokinesis. Encoded by the CDCA8 gene on chromosome 1p34.3, this protein interacts with Aurora B kinase, INCENP, and Survivin to form a functional complex that ensures accurate mitotic progression. Borealin is essential for proper kinetochore-microtubule attachment, spindle checkpoint activation, and correction of mitotic errors, thereby safeguarding genomic stability during cell division.

During mitosis, Borealin localizes to centromeres in metaphase and relocates to the spindle midzone during anaphase, coordinating the transition from chromosome alignment to cytokinesis. Loss of CDCA8 function results in chromosome missegregation, lagging chromatids, and multinucleation, phenotypes commonly associated with aneuploidy and cancer. Expression of CDCA8 peaks during the G2/M phase, reflecting its cell cycle-dependent regulation. Overexpression has been documented in a range of malignancies including breast, lung, and colorectal cancers, correlating with poor prognosis and high proliferative indices.

The CDCA8 antibody is widely used in cell cycle and cancer research to detect Borealin localization and expression dynamics. Immunofluorescence analysis with this antibody reveals distinct centromeric staining during metaphase and midbody localization in late mitosis. Western blotting identifies a 35 kilodalton band corresponding to the full-length protein. Because the chromosomal passenger complex regulates essential mitotic checkpoints, the CDCA8 antibody provides a critical tool for studying mechanisms of mitotic fidelity and tumorigenesis.

CDCA8 cooperates with Aurora B to phosphorylate substrates involved in spindle tension sensing and cytokinesis. Disruption of this interaction impairs CPC activity and leads to mitotic failure. Borealin's ability to bind DNA through its N-terminal domain and oligomerize via its coiled-coil region enables structural stability of the CPC. Inhibition or knockdown of CDCA8 sensitizes tumor cells to microtubule-targeting drugs, highlighting its potential as a therapeutic target. The antibody thus supports translational research exploring CPC inhibitors and mitotic checkpoint modulators in cancer therapy.

In developmental biology, CDCA8 is required for proper cell division during embryogenesis and organogenesis. The CDCA8 antibody facilitates identification of proliferating cells in rapidly dividing tissues and embryonic stem cell cultures. NSJ Bioreagents provides this antibody validated for its applications, ensuring consistent results in mitotic and oncogenic studies.

UniProt: Q53HL2

Pathways: Zellzyklus, M Phase