FEN1 Antikörper (AA 4-300)

Produktnummer ABIN7874666

Dieser Maus Monoklonal Antikörper detektiert spezifisch FEN1 in WB, IF, IHC (p) und FACS. Es zeigt Reaktivität gegenüber Proben von Human, Maus und Ratte.

Kurzübersicht für FEN1 Antikörper (AA 4-300) (ABIN7874666)

Target: FEN1 (Flap Structure-Specific Endonuclease 1 (FEN1))

Reaktivität: Human, Maus, Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser FEN1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS)

Klon: 6F3F2

Produktdetails anti-FEN1 Antikörper

Bindungsspezifität: AA 4-300

Verwendungszweck: Flap endonuclease 1 Antibody / FEN1

Aufreinigung: Antigen affinity purified

Immunogen: Recombinant human protein (amino acids Q4-E300) was used as the immunogen for the Flap endonuclease 1 antibody.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the Flap endonuclease 1 antibody should be determined by the researcher.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: After reconstitution, the Flap endonuclease 1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Details zu FEN1

Target: FEN1 (Flap Structure-Specific Endonuclease 1 (FEN1))

Andere Bezeichnung: Flap endonuclease 1

Hintergrund: Flap endonuclease 1 is an enzyme that in humans is encoded by the FEN1 gene. It is mapped to 11q12.2. The protein encoded by this gene removes 5' overhanging flaps in DNA repair and processes the 5' ends of Okazaki fragments in lagging strand DNA synthesis. Direct physical interaction between this protein and AP endonuclease 1 during long-patch base excision repair provides coordinated loading of the proteins onto the substrate, thus passing the substrate from one enzyme to another. The protein is a member of the XPG/RAD2 endonuclease family and is one of ten proteins essential for cell-free DNA replication. DNA secondary structure can inhibit flap processing at certain trinucleotide repeats in a length-dependent manner by concealing the 5' end of the flap that is necessary for both binding and cleavage by the protein encoded by this gene. Therefore, secondary structure can deter the protective function of this protein, leading to site-specific trinucleotide expansions.

UniProt: P39748

Pathways: Telomere Maintenance, DNA Reparatur, DNA Replication, Synthesis of DNA