POLM Antikörper (AA 36-481)

Produktnummer ABIN7874321

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch POLM in WB, ELISA und IP. Es zeigt Reaktivität gegenüber Proben von Human.

Kurzübersicht für POLM Antikörper (AA 36-481) (ABIN7874321)

Target: POLM (Polymerase (DNA Directed), mu (POLM))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser POLM Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Produktdetails anti-POLM Antikörper

Bindungsspezifität: AA 36-481

Verwendungszweck: POLM Antibody / DNA polymerase mu

Aufreinigung: Immunogen affinity purified

Immunogen: E.coli-derived human POLM recombinant protein (Position: E36-H481) was used as the immunogen for the POLM antibody.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the POLM antibody should be determined by the researcher.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: After reconstitution, the POLM antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Details zu POLM

Target: POLM (Polymerase (DNA Directed), mu (POLM))

Andere Bezeichnung: POLM

Hintergrund: POLM antibody detects DNA polymerase mu, a specialized DNA polymerase involved in non-homologous end joining (NHEJ) and DNA repair. The UniProt recommended name is DNA polymerase mu (POLM). This enzyme belongs to the X family of DNA polymerases, functioning at sites of double-strand breaks to fill in short gaps and process damaged DNA ends during repair.

Functionally, POLM antibody identifies a 494-amino-acid nuclear enzyme that exhibits template-dependent and template-independent polymerase activities. POLM incorporates nucleotides opposite damaged or missing bases, ensuring ligation competency of DNA ends. Unlike high-fidelity polymerases, POLM lacks strong proofreading activity, allowing it to tolerate mismatched or gapped templates-a property critical for repair flexibility in NHEJ.

The POLM gene is located on chromosome 7p13 and is expressed in immune and proliferative tissues such as thymus and bone marrow. It plays a key role in V(D)J recombination and class switch recombination in lymphocytes by repairing programmed double-strand breaks. Its activity complements DNA polymerase lambda (POLL) in filling in short DNA overhangs during repair.

Pathologically, dysregulation of POLM contributes to genomic instability and may influence cancer development, immune function, and cellular aging. Overexpression can increase mutation rates, whereas deficiency impairs NHEJ efficiency. POLM's balance between flexibility and error rate is vital for maintaining genome integrity while allowing adaptability in immune diversification.

POLM antibody is validated for western blotting, immunofluorescence, and immunohistochemistry to detect DNA polymerase mu in nuclear extracts. NSJ Bioreagents provides POLM antibody reagents optimized for DNA repair, recombination, and genomic maintenance research.

Structurally, DNA polymerase mu contains an N-terminal BRCT domain mediating protein-protein interactions, followed by a catalytic polymerase domain with palm, fingers, and thumb subdomains typical of X-family enzymes. This antibody supports studies on POLM's mechanistic role in end joining, mutation repair, and adaptive immunity.

UniProt: Q9NP87

Pathways: DNA Reparatur, Production of Molecular Mediator of Immune Response