LAT Antikörper (AA 28-235)

Produktnummer ABIN7873245

Der Kaninchen Polyklonal anti-LAT Antikörper wird verwendet zum Nachweis von LAT in Proben von Human. Er wurde validiert für WB, ELISA und FACS.

Kurzübersicht für LAT Antikörper (AA 28-235) (ABIN7873245)

Target: LAT (Linker For Activation of T Cells (LAT))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser LAT Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Produktdetails anti-LAT Antikörper

Bindungsspezifität: AA 28-235

Verwendungszweck: LAT Antibody / Linker for activation of T-cells family member 1

Aufreinigung: Immunogen affinity purified

Immunogen: E.coli-derived human LAT recombinant protein (Position: H28-E235) was used as the immunogen for the LAT antibody.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the LAT antibody should be determined by the researcher.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: After reconstitution, the LAT antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Details zu LAT

Target: LAT (Linker For Activation of T Cells (LAT))

Andere Bezeichnung: LAT

Hintergrund: LAT antibody detects Linker for activation of T-cells family member 1, encoded by the LAT gene. Linker for activation of T-cells family member 1 is a transmembrane adaptor protein expressed in T cells and natural killer cells, where it serves as a central scaffold for antigen receptor signaling. LAT antibody provides researchers with a critical reagent for studying T cell receptor signaling, immune responses, and related diseases.

Linker for activation of T-cells family member 1 localizes to lipid rafts in the plasma membrane. Research using LAT antibody has shown that upon T cell receptor stimulation, LAT becomes tyrosine-phosphorylated by ZAP70. These phosphorylated residues recruit multiple signaling proteins, including GRB2, PLCG1, and GADS, forming a large signaling complex. This assembly drives calcium signaling, MAPK activation, and transcription of immune effector genes.

Studies with LAT antibody have demonstrated that LAT is indispensable for T cell development and activation. Knockout models lacking LAT fail to develop mature T cells, and mutations in LAT impair signaling pathways required for adaptive immunity. These findings emphasize its role as a master regulator of immune receptor signaling.

Dysregulation of LAT contributes to disease. Research using LAT antibody has revealed that mutations in the LAT gene cause severe combined immunodeficiency, characterized by absent or impaired T cell function. Aberrant LAT signaling has also been implicated in autoimmune disease, where inappropriate activation leads to tissue damage. In oncology, altered LAT expression influences T cell activity within the tumor microenvironment, affecting anti-tumor immunity.

LAT antibody is widely used in immunoprecipitation, immunoblotting, and flow cytometry. Immunoprecipitation identifies signaling complexes assembled at LAT, immunoblotting detects phosphorylated and total protein levels, and flow cytometry quantifies expression in T cell populations. These applications make LAT antibody indispensable in immunology research.

By supplying validated LAT antibody reagents, NSJ Bioreagents supports studies into T cell receptor signaling, immune development, and pathology. Detection of Linker for activation of T-cells family member 1 provides researchers with insight into how scaffold proteins regulate receptor signaling networks.

UniProt: O43561

Pathways: T-Zell Rezeptor Signalweg, Fc-epsilon Rezeptor Signalübertragung