LGR5 Antikörper (AA 26-907)

Produktnummer ABIN7873020

Der Kaninchen Polyklonal anti-LGR5 Antikörper wird verwendet zum Nachweis von LGR5 in Proben von Human, Maus und Ratte. Er wurde validiert für WB, FACS, IHC und ELISA.

Kurzübersicht für LGR5 Antikörper (AA 26-907) (ABIN7873020)

Target: LGR5 (Leucine-Rich Repeat Containing G Protein-Coupled Receptor 5 (LGR5))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser LGR5 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (IHC), ELISA

Produktdetails anti-LGR5 Antikörper

Bindungsspezifität: AA 26-907

Verwendungszweck: LGR5 Antibody / Leucine-rich repeat-containing G-protein coupled receptor 5

Aufreinigung: Immunogen affinity purified

Immunogen: E.coli-derived human LGR5 recombinant protein (Position: R26-L907) was used as the immunogen for the LGR5 antibody.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the LGR5 antibody should be determined by the researcher.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: After reconstitution, the LGR5 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Details zu LGR5

Target: LGR5 (Leucine-Rich Repeat Containing G Protein-Coupled Receptor 5 (LGR5))

Andere Bezeichnung: LGR5

Hintergrund: LGR5 antibody detects Leucine-rich repeat-containing G-protein coupled receptor 5, a stem-cell marker and receptor for R-spondin proteins that potentiate Wnt/beta-catenin signaling. LGR5 is essential for maintaining self-renewing adult stem cell populations in the intestine, hair follicle, and stomach. The LGR5 antibody is used in stem-cell biology, oncology, and regenerative medicine to identify proliferative stem cell compartments.

LGR5 is encoded by the LGR5 gene on human chromosome 12q21.1. The receptor is approximately 907 amino acids long, with seven transmembrane domains typical of GPCRs and a large extracellular region containing leucine-rich repeats that mediate ligand binding. Upon R-spondin binding, LGR5 interacts with Frizzled and LRP5/6 receptors, amplifying Wnt signaling to sustain stem-cell proliferation.

The LGR5 antibody detects a 100-120 kilodalton protein by western blot and exhibits membrane and cytoplasmic localization in proliferative epithelial zones. In the intestine, LGR5 marks crypt base columnar cells capable of regenerating the entire epithelium. In the hair follicle, it labels bulge stem cells responsible for hair cycle renewal.

LGR5 expression is tightly regulated by Wnt activity and is a key marker for cancer stem cells in colorectal and gastric carcinomas. Overexpression promotes tumor initiation and therapy resistance, while knockdown suppresses proliferation and restores differentiation. The receptor modulates beta-catenin stabilization, transcription of target genes, and maintenance of undifferentiated states.

Because of its role in tissue regeneration and cancer stemness, LGR5 is an important biomarker for stem-cell tracking and targeted therapy development. NSJ Bioreagents provides a validated LGR5 antibody optimized for its applications, enabling precise identification of Wnt-responsive stem cell populations and cancer progenitors.

UniProt: O75473

Pathways: WNT Signalweg