APOA1 Antikörper (AA 25-264)

Produktnummer ABIN7872866

Dieser Maus Monoklonal Antikörper detektiert spezifisch APOA1 in WB und ELISA (Capture). Es zeigt Reaktivität gegenüber Proben von Human.

Kurzübersicht für APOA1 Antikörper (AA 25-264) (ABIN7872866)

Target: APOA1 (Apolipoprotein A-I (APOA1))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser APOA1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA (Capture)

Klon: 17G5

Produktdetails anti-APOA1 Antikörper

Bindungsspezifität: AA 25-264

Verwendungszweck: APOA1 Antibody / Apolipoprotein A

Aufreinigung: Affinity-chromatography

Immunogen: E. coli-derived mouse Apolipoprotein A I recombinant protein (Position: D25-Q264) was used as the immunogen for the APOA1 antibody.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the APOA1 antibody should be determined by the researcher.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: After reconstitution, the APOA1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Details zu APOA1

Target: APOA1 (Apolipoprotein A-I (APOA1))

Andere Bezeichnung: APOA1

Hintergrund: APOA1 antibody detects Apolipoprotein A-I, the major protein component of high-density lipoprotein (HDL) that mediates cholesterol transport and lipid metabolism. APOA1 facilitates reverse cholesterol transport by binding cellular cholesterol and phospholipids, delivering them to the liver for excretion. The APOA1 antibody is widely used in cardiovascular, metabolic, and lipid biology research to study HDL function, atherosclerosis prevention, and lipid-protein interactions.

APOA1 is encoded by the APOA1 gene located on human chromosome 11q23.3. The protein is approximately 243 amino acids long and synthesized mainly in the liver and small intestine. APOA1 circulates in plasma as part of HDL particles, where it activates lecithin-cholesterol acyltransferase (LCAT), an enzyme that esterifies free cholesterol, promoting HDL maturation. It plays a central role in maintaining lipid balance and preventing lipid accumulation in arterial walls.

The APOA1 antibody detects a 28 kilodalton band by western blot and exhibits strong cytoplasmic and extracellular staining in hepatic and intestinal tissues. APOA1 interacts with ATP-binding cassette transporters ABCA1 and ABCG1 to facilitate cholesterol efflux from peripheral cells. It also exhibits antioxidant and anti-inflammatory functions by neutralizing oxidized lipids and modulating cytokine signaling, contributing to cardiovascular protection.

Mutations or decreased expression of APOA1 lead to hypoalphalipoproteinemia and increased risk of coronary artery disease. Conversely, elevated APOA1 levels correlate with improved lipid clearance and reduced atherosclerotic plaque formation. In addition to lipid transport, APOA1 influences glucose metabolism, immune modulation, and amyloid aggregation prevention.

As a multifunctional lipid carrier and protective factor in cardiovascular health, APOA1 serves as both a biomarker and therapeutic target for metabolic disorders. NSJ Bioreagents provides a validated APOA1 antibody optimized for western blot, supporting research into HDL biology, cholesterol regulation, and cardiovascular disease prevention.

UniProt: P02647

Pathways: Regulation of Lipid Metabolism by PPARalpha, Production of Molecular Mediator of Immune Response, Lipid Metabolism