MBD4 Antikörper (AA 238-534)

Produktnummer ABIN7872642

Der Kaninchen Polyklonal anti-MBD4 Antikörper wird verwendet zum Nachweis von MBD4 in Proben von Maus und Ratte. Er wurde validiert für WB, ELISA und FACS.

Kurzübersicht für MBD4 Antikörper (AA 238-534) (ABIN7872642)

Target: MBD4 (Methyl-CpG Binding Domain Protein 4 (MBD4))

Reaktivität: Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser MBD4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Produktdetails anti-MBD4 Antikörper

Bindungsspezifität: AA 238-534

Verwendungszweck: Mbd4 Antibody / Methyl-CpG-binding domain protein 4

Aufreinigung: Immunogen affinity purified

Immunogen: E.coli-derived mouse Mbd4 recombinant protein (Position: R238-D534) was used as the immunogen for the Mbd4 antibody.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the Mbd4 antibody should be determined by the researcher.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: After reconstitution, the Mbd4 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Details zu MBD4

Target: MBD4 (Methyl-CpG Binding Domain Protein 4 (MBD4))

Andere Bezeichnung: Mbd4

Hintergrund: Mbd4 antibody detects Methyl-CpG-binding domain protein 4, a nuclear enzyme that couples DNA repair with epigenetic regulation by recognizing methylated DNA and mediating the excision of mismatched bases. Mbd4 functions as a bifunctional DNA glycosylase that specifically removes thymine or uracil mispaired with guanine within methylated CpG sites, thereby maintaining genomic stability and preventing mutation accumulation. The Mbd4 antibody is widely used in studies of DNA repair, epigenetics, and cancer biology to explore the connection between cytosine methylation and genome maintenance.

Mbd4 is encoded by the MBD4 gene located on human chromosome 3q21.3. The protein contains two main functional domains: an N-terminal methyl-CpG-binding domain (MBD) that targets methylated DNA, and a C-terminal glycosylase domain that catalyzes base excision. Through these domains, Mbd4 directly interacts with DNA methyltransferases and participates in active DNA demethylation pathways. It also forms complexes with MLH1, a mismatch repair factor, integrating base excision repair with the mismatch repair system.

The Mbd4 antibody typically detects a 63 kilodalton protein on western blot. Mbd4 is highly expressed in proliferative tissues and cells undergoing DNA replication. Functionally, loss of Mbd4 leads to increased mutation frequency at CpG sites, predisposing cells to carcinogenesis. Experimental models show that Mbd4-deficient mice accumulate G:T mismatches and are more susceptible to DNA damage induced by oxidative stress or alkylating agents.

Beyond repair, Mbd4 plays an important role in apoptosis and gene silencing. It interacts with the tumor suppressor p53 and participates in transcriptional repression of methylated gene promoters. Dysregulation of Mbd4 expression or mutations in its glycosylase domain have been linked to colorectal cancer, glioma, and leukemia, where defective base excision repair contributes to genomic instability. NSJ Bioreagents provides a validated Mbd4 antibody optimized for western blot, immunohistochemistry, and chromatin studies, supporting research on DNA methylation maintenance, repair mechanisms, and epigenetic disease progression.

UniProt: Q9Z2D7

Pathways: DNA Reparatur