NCOR1 Antikörper (AA 23-2075)

Produktnummer ABIN7872502

Der Kaninchen Polyklonal anti-NCOR1 Antikörper (ABIN7872502) detektiert spezifisch NCOR1 in ELISA, WB, IF, ICC und FACS. Dieser Antikörper reagiert spezifisch mit Proben aus Human.

Kurzübersicht für NCOR1 Antikörper (AA 23-2075) (ABIN7872502)

Target: NCOR1 (Nuclear Receptor Co-Repressor 1 (NCOR1))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser NCOR1 Antikörper ist unkonjugiert

Applikation: ELISA, Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Produktdetails anti-NCOR1 Antikörper

Bindungsspezifität: AA 23-2075

Verwendungszweck: NCOR1 Antibody / Nuclear receptor corepressor 1

Aufreinigung: Immunogen affinity purified

Immunogen: E.coli-derived human NCOR1 recombinant protein (Position: H23-Q2075) was used as the immunogen for the NCOR1 antibody.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the NCOR1 antibody should be determined by the researcher.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: After reconstitution, the NCOR1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Details zu NCOR1

Target: NCOR1 (Nuclear Receptor Co-Repressor 1 (NCOR1))

Andere Bezeichnung: NCOR1

Hintergrund: NCOR1 antibody detects Nuclear receptor corepressor 1, a transcriptional regulator that mediates repression of gene expression by nuclear hormone receptors and other transcription factors. The UniProt recommended name is Nuclear receptor corepressor 1 (NCOR1), with alternate names including nuclear receptor co-repressor, thyroid hormone receptor-interacting protein 13 (TRAC13), and retinoic acid receptor-binding protein. NCOR1 functions as a scaffold protein that bridges transcription factors with histone deacetylases (HDACs), leading to chromatin condensation and transcriptional silencing.

Functionally, NCOR1 antibody recognizes a large nuclear protein (~270 kDa) that forms complexes with histone deacetylase 3 (HDAC3) and transducin beta-like proteins (TBL1X, TBL1XR1), serving as the catalytic core of the NCOR/SMRT repression complex. NCOR1 regulates gene networks responsive to thyroid hormone, retinoic acid, glucocorticoids, and other ligands by recruiting HDAC3 to promoter regions. Through its receptor-interacting domains, NCOR1 binds unliganded nuclear receptors such as TR, RAR, and RXR to maintain basal transcriptional repression. Upon ligand binding, corepressors are released, and coactivators are recruited to enable gene activation.

In developmental and metabolic regulation, NCOR1 influences adipogenesis, gluconeogenesis, circadian rhythm, and immune cell differentiation. Deletion of NCOR1 in adipose tissue leads to increased mitochondrial activity and energy expenditure, highlighting its role in metabolic homeostasis. In neurons, NCOR1 modulates synaptic plasticity and behavior by repressing neural gene programs. Overexpression or mutation of NCOR1 can disrupt transcriptional balance and contribute to diseases including cancer, metabolic syndrome, and neurodevelopmental disorders. For example, NCOR1 loss correlates with enhanced oncogenic signaling in breast and prostate cancer models.

Structurally, NCOR1 contains multiple repression domains, nuclear receptor interaction motifs (L/IxxI/VI), and coiled-coil regions that enable interaction with HDAC3 and transcription factors. The NCOR1 antibody is widely used in chromatin immunoprecipitation (ChIP), western blotting, and immunofluorescence assays to examine chromatin binding, nuclear localization, and HDAC recruitment. The NCOR1 gene is located on chromosome 17p12 and encodes a 2453-amino acid protein conserved across mammals. It shares functional similarity with NCOR2 (SMRT), though the two exhibit distinct expression profiles and complex-specific functions.

By targeting a master regulator of transcriptional repression, NCOR1 antibody supports research in gene regulation, epigenetics, and signal-dependent chromatin remodeling. NSJ Bioreagents provides validated reagents for human, mouse, and rat NCOR1 detection across multiple experimental platforms for transcriptional and chromatin research.

UniProt: O75376

Pathways: Nuclear Hormone Receptor Binding, Chromatin Binding, Regulation of Lipid Metabolism by PPARalpha, Regulation of Carbohydrate Metabolic Process