TOP1MT Antikörper (AA 18-601)

Produktnummer ABIN7871431

Der Kaninchen Polyklonal anti-TOP1MT Antikörper (ABIN7871431) detektiert spezifisch TOP1MT in IHC, WB, FACS und ELISA. Dieser Antikörper reagiert spezifisch mit Proben aus Human und Maus.

Kurzübersicht für TOP1MT Antikörper (AA 18-601) (ABIN7871431)

Target: TOP1MT (Topoisomerase (DNA) I, Mitochondrial (TOP1MT))

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser TOP1MT Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC), Western Blotting (WB), Flow Cytometry (FACS), ELISA

Produktdetails anti-TOP1MT Antikörper

Bindungsspezifität: AA 18-601

Verwendungszweck: TOP1MT Antibody / DNA topoisomerase 1 mitochondrial

Aufreinigung: Immunogen affinity purified

Immunogen: E.coli-derived human TOP1MT recombinant protein (Position: E18-F601) was used as the immunogen for the TOP1MT antibody.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the TOP1MT antibody should be determined by the researcher.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: After reconstitution, the TOP1MT antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Details zu TOP1MT

Target: TOP1MT (Topoisomerase (DNA) I, Mitochondrial (TOP1MT))

Andere Bezeichnung: TOP1MT

Hintergrund: TOP1MT antibody detects DNA topoisomerase 1 mitochondrial, encoded by the TOP1MT gene. DNA topoisomerase 1 mitochondrial is an enzyme located in the mitochondrial matrix that relaxes supercoiled mitochondrial DNA, enabling replication, transcription, and repair. TOP1MT antibody provides researchers with a specific reagent for studying mitochondrial DNA metabolism, genome maintenance, and disease.

DNA topoisomerase 1 mitochondrial functions by introducing transient single-strand breaks in DNA, relieving torsional stress during replication and transcription. Research using TOP1MT antibody has shown that this enzyme maintains mitochondrial genome integrity by resolving supercoils generated during transcription of mitochondrial genes. Its activity is essential for proper mitochondrial gene expression and energy production.

Studies with TOP1MT antibody have revealed that loss of TOP1MT disrupts mitochondrial transcription and translation, impairing oxidative phosphorylation. This leads to reduced ATP generation, altered mitochondrial morphology, and increased oxidative stress. These phenotypes underscore its central role in mitochondrial biology.

Dysregulation of DNA topoisomerase 1 mitochondrial has been associated with human disease. Research using TOP1MT antibody has shown that variants can contribute to mitochondrial dysfunction, neuromuscular disorders, and cardiomyopathies. Furthermore, reduced TOP1MT activity exacerbates mitochondrial genome instability, linking it to degenerative and metabolic diseases.

Beyond mitochondrial maintenance, TOP1MT has been implicated in cellular stress responses and cancer. Research using TOP1MT antibody has shown that altered expression influences sensitivity to chemotherapy agents targeting topoisomerases, suggesting potential relevance in therapeutic response.

TOP1MT antibody is widely applied in immunohistochemistry, western blotting, and mitochondrial fractionation studies. Immunohistochemistry demonstrates mitochondrial localization, western blotting quantifies expression across tissues, and fractionation confirms enrichment in mitochondria. These applications make TOP1MT antibody indispensable in mitochondrial research.

By supplying validated TOP1MT antibody reagents, NSJ Bioreagents supports studies into mitochondrial genome regulation, oxidative phosphorylation, and disease. Detection of DNA topoisomerase 1 mitochondrial provides researchers with insight into how mitochondrial enzymes preserve genome stability and energy metabolism.

UniProt: Q969P6