TOP1MT Antikörper

Produktnummer ABIN6939147

Produktdetails anti-TOP1MT Antikörper

Target: TOP1MT (Topoisomerase (DNA) I, Mitochondrial (TOP1MT))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser TOP1MT Antikörper ist unkonjugiert

Applikation: Immunofluorescence (IF), Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Staining Methods (StM)

Aufreinigung: Purified by Protein A/G

Immunogen: Recombinant full-length human TOP1MT protein

Klon: TOP1MT-488

Isotyp: IgG2b kappa

Anwendungsinformationen

Applikationshinweise:

Positive Control: MCF-7, A431 cells. Jurkat cell lysate. Heart, Skeletal Muscle, Brain or Fetal Liver tissue.

Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (0.5-1 μg/mL), Western Blot (0.5-2 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-80 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Details zu TOP1MT

Target: TOP1MT (Topoisomerase (DNA) I, Mitochondrial (TOP1MT))

Andere Bezeichnung: TOP1MT (TOP1MT Produkte)

Synonyme: 2900052H09Rik antikoerper, TOP1 antikoerper, topoisomerase (DNA) I, mitochondrial antikoerper, DNA topoisomerase 1, mitochondrial antikoerper, DNA topoisomerase I mitochondrial antikoerper, TOP1MT antikoerper, Top1mt antikoerper

Hintergrund: DNA topoisomerases are nuclear enzymes that regulate the topological structure of DNA in eukaryotic cells by transiently breaking and rejoining DNA strands. Due to their roles in DNA replication, recombination, and transcription, DNA topoisomerases have been identified as targets of numerous anticancer drugs. Mitochondrial Topo I (DNA topoisomerase I, mitochondrial) is a 601 amino acid protein that primarily acts to relieve DNA strain that may occur during duplication of mitochondrial DNA. As a type IB topoisomerase, mitochondrial Topo I requires a divalent metal, either, calcium or magnesium, as well as an alkaline pH for optimal activity.

Molekulargewicht: 70kDa

Gen-ID: 116447

UniProt: Q969P6