GFR Antikörper (AA 164-574)

Produktnummer ABIN7871134

Der Kaninchen Polyklonal anti-GFR Antikörper (ABIN7871134) detektiert spezifisch GFR in WB und ELISA. Dieser Antikörper reagiert spezifisch mit Proben aus Human, Maus und Ratte.

Kurzübersicht für GFR Antikörper (AA 164-574) (ABIN7871134)

Target: GFR (RAPGEF5) (Rap Guanine Nucleotide Exchange Factor (GEF) 5 (RAPGEF5))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser GFR Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Produktdetails anti-GFR Antikörper

Bindungsspezifität: AA 164-574

Verwendungszweck: RAPGEF5 Antibody / Rap guanine nucleotide exchange factor 5

Aufreinigung: Immunogen affinity purified

Immunogen: E.coli-derived human RAPGEF5 recombinant protein (Position: E164-H574) was used as the immunogen for the RAPGEF5 antibody.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the RAPGEF5 antibody should be determined by the researcher.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: After reconstitution, the RAPGEF5 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Details zu GFR

Target: GFR (RAPGEF5) (Rap Guanine Nucleotide Exchange Factor (GEF) 5 (RAPGEF5))

Andere Bezeichnung: RAPGEF5

Hintergrund: RAPGEF5 antibody detects Rap guanine nucleotide exchange factor 5, a signaling protein that activates Rap1 and Rap2 small GTPases to regulate cell adhesion, polarity, and differentiation. The UniProt recommended name is Rap guanine nucleotide exchange factor 5 (RAPGEF5). This exchange factor converts inactive GDP-bound Rap GTPases into active GTP-bound forms, facilitating intracellular signaling cascades that govern morphogenesis and tissue organization.

Functionally, RAPGEF5 antibody identifies a 914-amino-acid cytoplasmic protein containing Ras exchange motif (REM) and CDC25 homology domains responsible for nucleotide exchange activity. RAPGEF5 is regulated by cyclic AMP and interacts with Rap-binding scaffolds to control cell junction assembly and migration. It acts as an important mediator of epithelial integrity and neural development.

The RAPGEF5 gene is located on chromosome 7p15.1 and is expressed in brain, lung, and epithelial tissues. Through Rap GTPase activation, RAPGEF5 coordinates cell-cell adhesion, endothelial barrier function, and axon guidance during development.

Pathologically, altered RAPGEF5 expression has been linked to cancer progression, vascular leakage, and neurological disorders. Dysregulation may lead to impaired Rap signaling, causing aberrant cell polarity and migration. Research using RAPGEF5 antibody supports studies in GTPase signaling, development, and cell adhesion regulation.

RAPGEF5 antibody is validated for western blotting, immunohistochemistry, and immunofluorescence to detect guanine nucleotide exchange factors. NSJ Bioreagents provides RAPGEF5 antibody reagents optimized for research in signaling, cytoskeletal regulation, and morphogenesis.

Structurally, Rap guanine nucleotide exchange factor 5 contains a cAMP-binding regulatory domain and catalytic regions that mediate activation of Rap GTPases. This antibody enables detailed analysis of RAPGEF5's contribution to intracellular signaling and polarity control.

UniProt: Q92565

Pathways: RTK Signalweg, Neurotrophin Signalübertragung, Hepatitis C