PLCD1 Antikörper (AA 14-549)

Produktnummer ABIN7870647

Der Kaninchen Polyklonal anti-PLCD1 Antikörper wird verwendet zum Nachweis von PLCD1 in Proben von Human, Maus und Ratte. Er wurde validiert für WB, ELISA und FACS.

Kurzübersicht für PLCD1 Antikörper (AA 14-549) (ABIN7870647)

Target: PLCD1 (phospholipase C, delta 1 (PLCD1))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PLCD1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Produktdetails anti-PLCD1 Antikörper

Bindungsspezifität: AA 14-549

Verwendungszweck: PLCD1 Antibody / Phospholipase C delta 1

Aufreinigung: Immunogen affinity purified

Immunogen: E.coli-derived human PLCD1 recombinant protein (Position: Q14-R549) was used as the immunogen for the PLCD1 antibody.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the PLCD1 antibody should be determined by the researcher.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: After reconstitution, the PLCD1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Details zu PLCD1

Target: PLCD1 (phospholipase C, delta 1 (PLCD1))

Andere Bezeichnung: PLCD1

Hintergrund: PLCD1 antibody detects Phospholipase C delta 1, a signaling enzyme that hydrolyzes phosphatidylinositol 4,5-bisphosphate (PIP2) into secondary messengers inositol trisphosphate (IP3) and diacylglycerol (DAG). The UniProt recommended name is Phospholipase C delta 1 (PLCD1). This enzyme participates in calcium signaling and regulates diverse cellular processes such as growth, differentiation, and cytoskeletal reorganization.

Functionally, PLCD1 antibody identifies a 756-amino-acid enzyme containing an N-terminal PH domain for membrane binding, EF-hand motifs for calcium interaction, a catalytic X-Y domain for phosphodiesterase activity, and a C2 domain for phospholipid association. Upon receptor stimulation, PLCD1 catalyzes the cleavage of PIP2, generating IP3 to release intracellular calcium and DAG to activate protein kinase C (PKC).

The PLCD1 gene is located on chromosome 3p22.3 and is expressed in epithelial tissues, brain, and smooth muscle. It functions downstream of receptor tyrosine kinases and G protein-coupled receptors to mediate signal transduction. PLCD1 contributes to actin cytoskeleton remodeling and cell migration through localized phosphoinositide metabolism.

Pathologically, downregulation or mutation of PLCD1 is associated with cancer progression, skin disorders, and developmental defects. PLCD1 acts as a tumor suppressor in certain epithelial cancers by modulating calcium signaling and cell adhesion. Research using PLCD1 antibody supports studies in signal transduction, lipid metabolism, and cell communication.

PLCD1 antibody is validated for western blotting, immunohistochemistry, and immunofluorescence to detect phospholipase enzymes in signaling pathways. NSJ Bioreagents provides PLCD1 antibody reagents optimized for cell signaling, cancer research, and phosphoinositide biology.

Structurally, Phospholipase C delta 1 contains catalytic and regulatory domains that coordinate substrate recognition and calcium-dependent activity. This antibody facilitates investigation of PLCD1's role in intracellular signaling and calcium homeostasis.

UniProt: P51178

Pathways: WNT Signalweg, Myometrial Relaxation and Contraction, Regulation of Carbohydrate Metabolic Process