ERCC5 Antikörper (AA 115-964)

Produktnummer ABIN7870213

Der Kaninchen Polyklonal anti-ERCC5 Antikörper (ABIN7870213) detektiert spezifisch ERCC5 in WB, ELISA, IHC, IF, ICC und FACS. Dieser Antikörper reagiert spezifisch mit Proben aus Human, Maus und Ratte.

Kurzübersicht für ERCC5 Antikörper (AA 115-964) (ABIN7870213)

Target: ERCC5 (DNA Repair Protein Complementing XP-G Cells (ERCC5))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser ERCC5 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Produktdetails anti-ERCC5 Antikörper

Bindungsspezifität: AA 115-964

Verwendungszweck: ERCC5 Antibody / Excision repair cross-complementation group 5

Aufreinigung: Immunogen affinity purified

Immunogen: E.coli-derived human XPG/ERCC5 recombinant protein (Position: K115-R964) was used as the immunogen for the ERCC5 antibody.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the ERCC5 antibody should be determined by the researcher.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: After reconstitution, the ERCC5 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Details zu ERCC5

Target: ERCC5 (DNA Repair Protein Complementing XP-G Cells (ERCC5))

Andere Bezeichnung: ERCC5

Hintergrund: ERCC5 antibody detects Excision repair cross-complementation group 5 protein, a structure-specific endonuclease essential for nucleotide excision repair (NER). Also known as Xeroderma pigmentosum group G protein (XPG), ERCC5 catalyzes 3' incision at sites of DNA damage, removing bulky lesions such as ultraviolet-induced pyrimidine dimers. The ERCC5 antibody is widely used in DNA repair, cancer, and dermatological research to study genome stability, DNA damage responses, and repair-deficient disorders.

ERCC5 is encoded by the ERCC5 gene located on human chromosome 13q33.1. The protein is approximately 118 kilodaltons in size and belongs to the XPG/RAD2 family of structure-specific nucleases. It contains conserved N- and I-domains forming the catalytic core, as well as C-terminal regions mediating interactions with transcription and repair factors such as TFIIH and PCNA. Through these interactions, ERCC5 coordinates incision with repair synthesis during the NER process.

The ERCC5 antibody typically detects a strong nuclear band near 133 kilodaltons by western blot. Immunofluorescence reveals nuclear localization, often enriched at DNA damage foci following UV or oxidative stress exposure. Functional studies demonstrate that ERCC5 excises damaged DNA downstream of the lesion, allowing DNA polymerases to resynthesize the correct strand. Mutations in ERCC5 cause Xeroderma pigmentosum complementation group G (XP-G) and Cockayne syndrome, conditions characterized by UV hypersensitivity, neurological abnormalities, and premature aging.

Beyond its canonical repair function, ERCC5 also contributes to transcription-coupled repair and RNA polymerase II-mediated gene expression. It participates in DNA replication stress responses and protects replication forks from collapse under genotoxic stress. Dysregulation or reduced expression of ERCC5 has been linked to increased cancer susceptibility, particularly skin, liver, and lung cancers. NSJ Bioreagents offers a validated ERCC5 antibody optimized for western blot, immunohistochemistry, and DNA damage studies, providing researchers with a reliable tool for analyzing DNA repair mechanisms, NER efficiency, and genome protection pathways.

UniProt: P28715

Pathways: DNA Reparatur