LIG4 Antikörper (AA 1-876)

Produktnummer ABIN7869599

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch LIG4 in WB, ELISA, IF, ICC und FACS. Es zeigt Reaktivität gegenüber Proben von Human, Maus und Ratte.

Kurzübersicht für LIG4 Antikörper (AA 1-876) (ABIN7869599)

Target: LIG4 (Ligase IV, DNA, ATP-Dependent (LIG4))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser LIG4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Produktdetails anti-LIG4 Antikörper

Bindungsspezifität: AA 1-876

Verwendungszweck: LIG4 Antibody / DNA Ligase 4

Aufreinigung: Immunogen affinity purified

Immunogen: E.coli-derived human DNA Ligase IV/LIG4 recombinant protein (Position: M1-K876) was used as the immunogen for the LIG4 antibody.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the LIG4 antibody should be determined by the researcher.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: After reconstitution, the LIG4 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Details zu LIG4

Target: LIG4 (Ligase IV, DNA, ATP-Dependent (LIG4))

Andere Bezeichnung: LIG4

Hintergrund: LIG4 antibody detects DNA ligase 4, an essential enzyme that catalyzes the final ligation step in non-homologous end joining (NHEJ) DNA repair. The UniProt recommended name is DNA ligase 4 (LIG4). This enzyme joins DNA double-strand breaks generated during recombination, replication stress, or DNA damage, preserving genome stability.

Functionally, LIG4 antibody identifies a 911-amino-acid ATP-dependent DNA ligase that seals nicks in double-stranded DNA by catalyzing phosphodiester bond formation. LIG4 operates in complex with XRCC4 and XLF/Cernunnos to align DNA ends and complete repair during NHEJ. It also plays a critical role in V(D)J recombination in lymphocytes, supporting immune diversity.

The LIG4 gene is located on chromosome 13q33.3 and is ubiquitously expressed in proliferating tissues. Its expression is highest in immune cells, where DNA recombination occurs frequently. Proper function of LIG4 ensures efficient DNA repair and prevents chromosomal translocations and mutagenesis.

Pathologically, mutations in LIG4 cause Ligase IV syndrome, characterized by microcephaly, developmental delay, immunodeficiency, and radiosensitivity. Deficiency in LIG4 impairs DNA double-strand break repair, leading to genomic instability and increased cancer susceptibility. Research using LIG4 antibody aids in studies of DNA repair, recombination, and genomic maintenance.

LIG4 antibody is validated for western blotting, immunohistochemistry, and immunofluorescence to detect ligase enzymes in DNA damage response pathways. NSJ Bioreagents provides LIG4 antibody reagents optimized for DNA repair, oncology, and immunology research.

Structurally, DNA ligase 4 contains an N-terminal DNA-binding domain, an adenylation domain that binds AMP, and a C-terminal XRCC4-interacting region necessary for NHEJ complex formation. This antibody enables detailed analysis of LIG4's enzymatic activity in genome integrity and repair pathways.

UniProt: P49917

Pathways: DNA Reparatur, Production of Molecular Mediator of Immune Response