MGMT Antikörper (AA 1-100)

Produktnummer ABIN7867877

Dieser Kaninchen Monoklonal Antikörper detektiert spezifisch MGMT in IHC (p). Es zeigt Reaktivität gegenüber Proben von Human.

Kurzübersicht für MGMT Antikörper (AA 1-100) (ABIN7867877)

Target: MGMT (O6-Methylguanine-DNA-Methyltransferase (MGMT))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Monoklonal

Konjugat: Dieser MGMT Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Güteklasse: Carrier-free

Klon: MGMT-8128R

Produktdetails anti-MGMT Antikörper

Bindungsspezifität: AA 1-100

Verwendungszweck: MGMT Antibody (azide and preservative free)

Aufreinigung: Protein A/G affinity

Immunogen: A recombinant partial protein (within amino acids 1-100) from the human protein was used as the immunogen for the MGMT antibody.

Isotyp: IgG, kappa

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the MGMT antibody should be determined by the researcher.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Konservierungsmittel: Azide free

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot the MGMT antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Details zu MGMT

Target: MGMT (O6-Methylguanine-DNA-Methyltransferase (MGMT))

Andere Bezeichnung: MGMT

Hintergrund: Cancer chemotherapeutic alkylating agents (e.g. BCNU,) act by inducing formation of lethal cross-links at the O6- alkylguanine position in DNA. MGMT transfers alkyl adducts from the O6- position of guanine in DNA (prior to cross-link formation) to a cysteine residue in its own sequence, thereby restoring DNA to its intact state. This transfer inactivates the MGMT enzyme and is irreversible, hence the level of MGMT in a cell is directly proportional to the level of DNA damage it can tolerate. In normal tissues, MGMT acts as a suppressor of mutation and carcinogenesis. Tumors with high levels of MGMT are likely to be drug resistant.

UniProt: P16455

Pathways: DNA Reparatur, Positive Regulation of Response to DNA Damage Stimulus