Retinoblastoma 1 Antikörper (pThr826)

Produktnummer ABIN7862502

Der Kaninchen Polyklonal anti-Retinoblastoma 1 Antikörper wird verwendet zum Nachweis von Retinoblastoma 1 in Proben von Human, Maus und Ratte. Er wurde validiert für WB, IHC, ELISA und IF.

Kurzübersicht für Retinoblastoma 1 Antikörper (pThr826) (ABIN7862502)

Target: Retinoblastoma 1 (RB1)

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Retinoblastoma 1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Produktdetails anti-Retinoblastoma 1 Antikörper

Bindungsspezifität: pThr826

Verwendungszweck: Rabbit Anti-Rb (phospho Thr826) Polyclonal Antibody

Spezifität: Phospho-Rb (T826) Polyclonal Antibody detects endogenous levels of Rb protein only when phosphorylated at T826.

Aufreinigung: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human Rb Phospho-Thr826

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,IF 1:200-1:1000,ELISA 1:10000,Not yet tested in other applications.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Haltbarkeit: 12 months

Details zu Retinoblastoma 1

Target: Retinoblastoma 1 (RB1)

Andere Bezeichnung: Rb

Hintergrund: RB1, Retinoblastoma-associated protein, p105-Rb, pRb, Rb, pp110The protein encoded by RB1 (RB transcriptional corepressor 1) is a negative regulator of the cell cycle and was the first tumor suppressor gene found. The encoded protein also stabilizes constitutive heterochromatin to maintain the overall chromatin structure. The active, hypophosphorylated form of the protein binds transcription factor E2F1. Defects in RB1 are a cause of childhood cancer retinoblastoma (RB), bladder cancer, and osteogenic sarcoma.

Molekulargewicht: 110kD

Gen-ID: 5925

UniProt: P06400

Pathways: Zellzyklus, Intracellular Steroid Hormone Receptor Signaling Pathway, Mitotic G1-G1/S Phases, DNA Replication, Maintenance of Protein Location, Synthesis of DNA, Autophagie