Retinoblastoma 1 Antikörper (pSer807)

Produktnummer ABIN7862497

Der Kaninchen Polyklonal anti-Retinoblastoma 1 Antikörper (ABIN7862497) detektiert spezifisch Retinoblastoma 1 in WB, IHC, ELISA und IF. Dieser Antikörper reagiert spezifisch mit Proben aus Human, Maus und Ratte.

Kurzübersicht für Retinoblastoma 1 Antikörper (pSer807) (ABIN7862497)

Target: Retinoblastoma 1 (RB1)

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Retinoblastoma 1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Produktdetails anti-Retinoblastoma 1 Antikörper

Bindungsspezifität: pSer807

Verwendungszweck: Rabbit Anti-Rb (phospho Ser807) Polyclonal Antibody

Spezifität: Phospho-Rb (S807) Polyclonal Antibody detects endogenous levels of Rb protein only when phosphorylated at S807.

Aufreinigung: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human Rb Phospho-Ser807

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,ELISA 1:10000,IF 1:50-200

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Haltbarkeit: 12 months

Details zu Retinoblastoma 1

Target: Retinoblastoma 1 (RB1)

Andere Bezeichnung: Rb

Hintergrund: RB1, Retinoblastoma-associated protein, p105-Rb, pRb, Rb, pp110RB transcriptional corepressor 1 encoded by RB1 is a negative regulator of the cell cycle and was the first tumor suppressor gene found. RB transcriptional corepressor 1 also stabilizes constitutive heterochromatin to maintain the overall chromatin structure. The active, hypophosphorylated form of the protein binds transcription factor E2F1. Defects in RB1 are a cause of childhood cancer retinoblastoma (RB), bladder cancer, and osteogenic sarcoma.

Molekulargewicht: 106kD

Gen-ID: 5925

UniProt: P06400

Pathways: Zellzyklus, Intracellular Steroid Hormone Receptor Signaling Pathway, Mitotic G1-G1/S Phases, DNA Replication, Maintenance of Protein Location, Synthesis of DNA, Autophagie