Rekombinanter Retinoblastoma 1 Antikörper

Produktnummer ABIN7862004

Der Maus Monoklonal anti-Retinoblastoma 1 Antikörper wird verwendet zum Nachweis von Retinoblastoma 1 in Proben von Human und Maus. Er wurde validiert für WB und IHC.

Kurzübersicht für Rekombinanter Retinoblastoma 1 Antikörper (ABIN7862004)

Target: Retinoblastoma 1 (RB1)

Antikörpertyp: Recombinant Antibody

Reaktivität: Human, Maus

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Retinoblastoma 1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC)

Klon: RRB1-8060

Produktdetails anti-Retinoblastoma 1 Antikörper

Verwendungszweck: Recombinant Rb1 (Tumor Suppressor Protein) Antibody

Spezifität: Recognizes a 105 kDa phosphoprotein, identified as retinoblastoma (Rb) gene product. Its epitope is localized between aa 703-772. It shows no cross reaction with p107 or p130. It specifically stains the nuclei of BT-20 cells and primary human foreskin fibroblast (HFF) cells. It does not stain the Rb-negative BT549 cells. It reacts with the hyperphosphorylated as well as the un (under) phosphorylated form of the Rb protein. Retinoblastoma gene product plays a key role in cell cycle control. It has been identified as a tumor suppressor gene whose loss of its function leads to tumor development. It is widely expressed in a variety of human tissues including breast, esophageal, squamous cell and cervical carcinoma.

Aufreinigung: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant human Rb1 protein fragment of 283 amino acid residues.

Isotyp: IgG1, kappa

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Konservierungsmittel: Azide free

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C.Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Details zu Retinoblastoma 1

Target: Retinoblastoma 1 (RB1)

Andere Bezeichnung: Rb1

Hintergrund:

OSRC, Osteosarcoma, p105-Rb, PP105, pp110, pRb, Prepro retinoblastoma associated protein, RB1, Retinoblastoma 1, Retinoblastoma-associated protein

A431 or BT-20 cells. Human breast or colon carcinoma.|HeLa, Intestine, Kidney, Lung, Skin, Spleen.

Molekulargewicht: 105kDa

Gen-ID: 5925, 408528

UniProt: P06400

Pathways: Zellzyklus, Intracellular Steroid Hormone Receptor Signaling Pathway, Mitotic G1-G1/S Phases, DNA Replication, Maintenance of Protein Location, Synthesis of DNA, Autophagie