MGMT Antikörper

Produktnummer ABIN7861877

Dieser Maus Monoklonal Antikörper detektiert spezifisch MGMT in WB und IHC. Es zeigt Reaktivität gegenüber Proben von Human.

Kurzübersicht für MGMT Antikörper (ABIN7861877)

Target: MGMT (O6-Methylguanine-DNA-Methyltransferase (MGMT))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser MGMT Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC)

Klon: MGMT-7454

Produktdetails anti-MGMT Antikörper

Verwendungszweck: MGMT (Marker of Suppressor of Mutation and Carcinogenesis) Antibody

Spezifität: Cancer chemotherapeutic alkylating agents (e.g. BCNU,) act by inducing formation of lethal cross 79oΩlinks at the O6 79oΩ alkylguanine position in DNA. MGMT transfers alkyl adducts from the O6 79oΩposition of guanine in DNA (prior to cross 79oΩlink formation) to a cysteine residue in its own sequence, thereby restoring DNA to its intact state. This transfer inactivates the MGMT enzyme and is irreversible, hence the level of MGMT in a cell is directly proportional to the level of DNA 79oΩdamage it can tolerate. In normal tissues, MGMT acts as a suppressor of mutation and carcinogenesis. Tumors with high levels of MGMT are likely to be drug resistant

Aufreinigung: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Purified His-tagged MGMT protein

Isotyp: IgG2, kappa

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Konservierungsmittel: Azide free

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C.Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Details zu MGMT

Target: MGMT (O6-Methylguanine-DNA-Methyltransferase (MGMT))

Andere Bezeichnung: MGMT

Hintergrund:

6 O methylguanine DNA methyltransferase, Methylated DNA protein cysteine methyltransferase, O 6 methylguanine DNA alkyltransferase, O 6 methylguanine DNA methyltransferase

Jurkat , MCF-7, LNCaP, Hep G2 and human liver. Human tonsil or stomach (IHC). Human liver, Human pancreas or human skeletal muscle

Molekulargewicht: 21.646 kDa

Gen-ID: 4255, 501522

Pathways: DNA Reparatur, Positive Regulation of Response to DNA Damage Stimulus