Rekombinanter Keratin Acidic (AE1) Antikörper

Produktnummer ABIN7861617

Der Maus Monoklonal anti-Keratin Acidic Antikörper wird verwendet zum Nachweis von Keratin Acidic in Proben von Human, Ratte, Huhn, Rind (Kuh), Maus, Kaninchen, Affe, Hund und Schildkröte. Er wurde validiert für IHC und WB.

Kurzübersicht für Rekombinanter Keratin Acidic (AE1) Antikörper (ABIN7861617)

Target: Keratin Acidic (AE1)

Antikörpertyp: Recombinant Antibody

Reaktivität: Human, Ratte, Huhn, Rind (Kuh), Maus, Kaninchen, Affe, Hund, Schildkröte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Unkonjugiert

Applikation: Immunohistochemistry (IHC), Western Blotting (WB)

Klon: RKRTL-8751

Produktdetails

Verwendungszweck: Recombinant Cytokeratin, Acidic (Type I or LMW) (Epithelial Marker) Antibody

Spezifität: This MAb recognizes the 56.5 kDa (CK10), 50 kDa (CK14), 50 kDa (CK15), 48 kDa (CK16), 40 kDa (CK19) keratins of the acidic (Type I or LMW) subfamily. Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. The acidic keratins have molecular weights (MW) of 56.5, 55, 51, 50, 50 , 48, 46, 45, and 40 kDa. MAb AE3 recognizes the 65-67, 64, 59, 58, 56, and 52 kDa keratins of basic subfamily. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis. AE1/AE3 is a broad spectrum anti pan-keratin antibody cocktail, which differentiates epithelial tumors from non-epithelial tumors e.g. squamous vs. adenocarcinoma of the lung, liver carcinoma, breast cancer, and esophageal cancer.

Aufreinigung: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Human epidermal keratin

Isotyp: IgG1, kappa

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C.Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Antigendetails

Target: Keratin Acidic (AE1)

Andere Bezeichnung: Cytokeratin, Acidic

Hintergrund:

K1B, KRT1B, Keratin, type II cytoskeletal 1b, K77, CK-1B, Keratin 1B, Keratin-77, Cytokeratin-1B, Type-II Keratin Kb39

Skin or squamous cell carcinoma (IHC). A431.

Molekulargewicht: 40-56.5kDa

Gen-ID: 374454, 334989