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Rekombinanter Keratin Acidic (AE1) Antikörper (CF®488A)

AE1 Reaktivität: Human IHC, IF, WB, FACS Wirt: Kaninchen Monoclonal KRTL-1577R CF®488A Recombinant Antibody
Produktnummer ABIN6178766
  • Target Alle Keratin Acidic (AE1) Antikörper anzeigen
    Keratin Acidic (AE1)
    Antikörpertyp
    Recombinant Antibody
    Reaktivität
    • 31
    • 11
    • 9
    • 9
    • 9
    • 7
    • 6
    • 5
    • 4
    • 3
    • 2
    • 2
    Human
    Wirt
    • 18
    • 10
    • 3
    Kaninchen
    Klonalität
    • 31
    Monoklonal
    Konjugat
    • 15
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    CF®488A
    Applikation
    • 23
    • 23
    • 23
    • 19
    • 12
    • 3
    • 3
    • 2
    Immunohistochemistry (IHC), Immunofluorescence (IF), Western Blotting (WB), Flow Cytometry (FACS)
    Verwendungszweck
    Recombinant Rabbit Monoclonal anti-Cytokeratin, Acidic (KRTL/1577R), CF488A Conjugate
    Produktmerkmale
    This MAb recognizes the 56.5 kDa (CK10), 50 kDa (CK14), 50 kDa (CK15), 48 kDa (CK16), 40 kDa (CK19) keratins of the acidic (Type I or LMW) subfamily. Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI <5.7) and basic (pI >6.0) subfamilies. The acidic keratins have molecular weights (MW) of 56.5, 55, 51, 50, 50 , 48, 46, 45, and 40 kDa. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
    Immunogen
    Recombinant human KRT77 protein fragment
    Klon
    KRTL-1577R
    Isotyp
    IgG kappa
    Top Product
    Discover our top product AE1 Primärantikörper
  • Applikationshinweise
    Immunohistology (formalin) 1-2 μg/mL
    • Immunofluorescence 1-2 μg/mL Western blotting 0.5-1 μg/mL
    • Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min
    • Flow Cytometry 0.5-1 μg/million cells/0.1 mL
    • Optimal dilution for a specific application should be determined by user
    Kommentare

    Skin, Squamous cell carcinoma (SCC)

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Konzentration
    100 μg/mL
    Buffer
    PBS/0.1 % BSA/0.05 % azide
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handhabung
    Protect from light
  • Target
    Keratin Acidic (AE1)
    Andere Bezeichnung
    Cytokeratin, Acidic (AE1 Produkte)
    Hintergrund
    K1B, KRT1B, K77, CK-1B, Keratin 1B, Keratin-77, Cytokeratin-1B
    Molekulargewicht
    56.5 kDa (CK10), 50 kDa (CK14), 50 kDa (CK15), 48 kDa (CK16), 40 kDa (CK19)
    Gen-ID
    374454, 334989
    UniProt
    Q7Z794
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