Crossover junction endonuclease EME1 (EME1) Antikörper

Produktnummer ABIN7862672

Der Maus Monoklonal anti-Crossover junction endonuclease EME1 Antikörper (Klon MTA31 7H2-1) (ABIN7862672) detektiert spezifisch Crossover junction endonuclease EME1 in WB, IHC und IF. Dieser Antikörper reagiert spezifisch mit Proben aus Human.

Kurzübersicht für Crossover junction endonuclease EME1 (EME1) Antikörper (ABIN7862672)

Target: Crossover junction endonuclease EME1 (EME1)

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Klon: MTA31 7H2-1

Produktdetails

Verwendungszweck: EME1 Antibody

Spezifität: Essential meiotic endonuclease 1 (Eme1), a member of the Eme1/Mms4 family, associates with Mus81 to constitute a heterodimeric endonuclease that has been implicated in mitotic and meiotic recombination in eukaryotes. The Mus81-Eme1 complex cleaves branched DNA structures, especially those arising during stalled DNA replication such as replication forks and 3' DNA flaps. When purified from yeast, this complex cleaves synthetic Holliday junctions into linear duplex DNA. These findings provide compelling evidence that Mus81-Eme1 complexes are essential elements of the eukaryotic nuclear Holliday junction resolvase. Eme1 may also be required in mitosis for the processing of collapsed replication forks. Eme1 is typically localized to the nucleolus and is recruited to regions of DNA damage in S phase cells.

Aufreinigung: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: His-tagged recombinant Eme1 of human origin

Isotyp: IgG1, kappa

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Antigendetails

Target: Crossover junction endonuclease EME1 (EME1)

Andere Bezeichnung: EME1

Hintergrund:

Crossover junction endonuclease EME1, Essential Meiotic Endonuclease 1 Homolog 1/2, hMMS4, MMS4L, SLX2A

HEK293T or U-87 MG cells. Human tonsil.

Molekulargewicht: 65kDa

Gen-ID: 146956, 514330

UniProt: Q96AY2