Crossover junction endonuclease EME1 (EME1) Antikörper
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- Target Alle Crossover junction endonuclease EME1 (EME1) Antikörper anzeigen
- Crossover junction endonuclease EME1 (EME1)
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Reaktivität
- Human
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Wirt
- Maus
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Klonalität
- Monoklonal
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Konjugat
- Unkonjugiert
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Applikation
- Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunoprecipitation (IP)
- Spezifität
- Essential meiotic endonuclease 1 (Eme1), a member of the Eme1/Mms4 family, associates with Mus81 to constitute a heterodimeric endonuclease that has been implicated in mitotic and meiotic recombination in eukaryotes. The Mus81-Eme1 complex cleaves branched DNA structures, especially those arising during stalled DNA replication such as replication forks and 3' DNA flaps. When purified from yeast, this complex cleaves synthetic Holliday junctions into linear duplex DNA. These findings provide compelling evidence that Mus81-Eme1 complexes are essential elements of the eukaryotic nuclear Holliday junction resolvase. Eme1 may also be required in mitosis for the processing of collapsed replication forks. Eme1 is typically localized to the nucleolus and is recruited to regions of DNA damage in S phase cells.
- Kreuzreaktivität (Details)
- Human.
- Aufreinigung
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
- Immunogen
- His-tagged recombinant Eme1 of human origin
- Isotyp
- IgG1
- Top Product
- Discover our top product EME1 Primärantikörper
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- Applikationshinweise
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Known_Application: Immunoprecipitation (1-2 μg per 100-500 μg of total protein (1 mL of cell lysate)), ,Western Blot (1-2 μg/mL), Immunofluorescence (1-2 μg/mL), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
Positive_Control: HEK293T or U-87 MG cells. Human tonsil.
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Konzentration
- 1.0 mg/mL
- Buffer
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
- Konservierungsmittel
- Azide free
- Lagerung
- -20 °C,-80 °C
- Informationen zur Lagerung
- Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
- Haltbarkeit
- 24 months
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- Target
- Crossover junction endonuclease EME1 (EME1)
- Andere Bezeichnung
- EME1 (EME1 Produkte)
- Synonyme
- 6820428D13 antikoerper, essential meiotic structure-specific endonuclease 1 antikoerper, Eme1 antikoerper
- Hintergrund
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Crossover junction endonuclease EME1, Essential Meiotic Endonuclease 1 Homolog 1/2, hMMS4, MMS4L, SLX2A,EME1
Cellular localisation: Nucleus - Molekulargewicht
- 65kDa
- Gen-ID
- 146956, 514330
- UniProt
- Q96AY2
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