Nanog Antikörper (AA 100-250)

Produktnummer ABIN7865247

Der Maus Monoklonal anti-Nanog Antikörper (Klon NANOG-9704) (ABIN7865247) detektiert spezifisch Nanog in IHC. Dieser Antikörper reagiert spezifisch mit Proben aus Human.

Kurzübersicht für Nanog Antikörper (AA 100-250) (ABIN7865247)

Target: Nanog (NANOG) (Nanog Homeobox (NANOG))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Nanog Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC)

Klon: NANOG-9704

Produktdetails anti-Nanog Antikörper

Bindungsspezifität: AA 100-250

Verwendungszweck: Homeobox protein NANOG (Cancer Stem Cell Marker) Antibody

Spezifität: The homeobox protein Nanog is a transcription factor crucial for maintaining the pluripotency of embryonic stem cells (ESCs) by inhibiting cell differentiation factors. In humans, the NANOG gene encodes this protein. Nanog operates alongside other factors such as Oct-4 and SOX2 to define ESC identity. It is also highly expressed in cancer stem cells, suggesting a potential role as an oncogene in promoting cancer development. Elevated levels of Nanog are linked to poor prognosis in cancer patients. Nanog shows strong and specific expression in carcinoma in situ (CIS), embryonal carcinomas, and seminomas but are absent in teratomas and yolk sac tumors. Research indicates that human embryonic stem cell-related genes, including OCT4, NANOG, STELLAR, and GDF3, are expressed in seminomas and breast carcinomas. Nanog positivity is notably associated with high-grade ovarian serous carcinoma but is not observed in benign, borderline, or low-grade serous lesions. One study highlights the cellular shuttling of Nanog and its increased stromal presence during cervical cancer progression. Furthermore, Nanog overexpression has been linked to factors such as tumor differentiation, lymph node metastasis, and tumor size, with studies suggesting its predictive value for reduced overall survival (OS) and disease-free survival (DFS) in lung cancer.

Aufreinigung: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant fragment (around aa100-250) of human NANOG protein (exact sequence is proprietary)

Isotyp: IgG2b, lambda

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C.Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Details zu Nanog

Target: Nanog (NANOG) (Nanog Homeobox (NANOG))

Andere Bezeichnung: Homeobox protein NANOG

Hintergrund:

homeobox transcription factor Nanog, homeobox transcription factor Nanog-delta 48

Human testicular carcinoma.

Molekulargewicht: 40kDa

Gen-ID: 79923

UniProt: Q9H9S0

Pathways: Stem Cell Maintenance