Rekombinanter MGMT Antikörper (AA 1-100)

Produktnummer ABIN7864246

Dieser Kaninchen Monoklonal Antikörper detektiert spezifisch MGMT in WB und IHC. Es zeigt Reaktivität gegenüber Proben von Human.

Kurzübersicht für Rekombinanter MGMT Antikörper (AA 1-100) (ABIN7864246)

Target: MGMT (O6-Methylguanine-DNA-Methyltransferase (MGMT))

Antikörpertyp: Recombinant Antibody

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Monoklonal

Konjugat: Dieser MGMT Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC)

Klon: MGMT-8364R

Produktdetails anti-MGMT Antikörper

Bindungsspezifität: AA 1-100

Verwendungszweck: Recombinant MGMT (Marker of Suppressor of Mutation and Carcinogenesis) Antibody

Spezifität: Cancer chemotherapeutic alkylating agents (e.g. BCNU,) act by inducing formation of lethal crossT 79oΩ 79oΩlinks at the O6T 79oΩ 79oΩ alkylguanine position in DNA. MGMT transfers alkyl adducts from the O6T 79oΩ 79oΩposition of guanine in DNA (prior to crossT 79oΩ 79oΩlink formation) to a cysteine residue in its own sequence, thereby restoring DNA to its intact state. This transfer inactivates the MGMT enzyme and is irreversible, hence the level of MGMT in a cell is directly proportional to the level of DNAT 79oΩ 79oΩdamage it can tolerate. In normal tissues, MGMT acts as a suppressor of mutation and carcinogenesis. Tumors with high levels of MGMT are likely to be drug resistant

Aufreinigung: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant fragment (around aa1-100) of human MGMT (exact sequence is proprietary)

Isotyp: IgG, kappa

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Details zu MGMT

Target: MGMT (O6-Methylguanine-DNA-Methyltransferase (MGMT))

Andere Bezeichnung: MGMT

Hintergrund:

6 O methylguanine DNA methyltransferase, Methylated DNA protein cysteine methyltransferase, O 6 methylguanine DNA alkyltransferase, O 6 methylguanine DNA methyltransferase

Jurkat, MCF-7, LNCaP, Hep G2 and human liver. Human tonsil or stomach (IHC).

Molekulargewicht: 21.646 kDa

Gen-ID: 4255, 501522

UniProt: P16455

Pathways: DNA Reparatur, Positive Regulation of Response to DNA Damage Stimulus