Rekombinanter Choline Acetyltransferase Antikörper (C-Term)

Produktnummer ABIN7865483

Der Maus Monoklonal anti-Choline Acetyltransferase Antikörper wird verwendet zum Nachweis von Choline Acetyltransferase in Proben von Human. Er wurde validiert für IHC.

Kurzübersicht für Rekombinanter Choline Acetyltransferase Antikörper (C-Term) (ABIN7865483)

Target: Choline Acetyltransferase (CHAT) (Choline O-Acetyltransferase (CHAT))

Antikörpertyp: Recombinant Antibody

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Choline Acetyltransferase Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC)

Klon: R38B12

Produktdetails anti-Choline Acetyltransferase Antikörper

Bindungsspezifität: C-Term

Expressionssystem: CHO Cells

Verwendungszweck: Recombinant Choline Acetyltransferase (CHAT) (Marker of Cholinergic Cells) Antibody

Spezifität: Choline acetyltransferase (ChAT) is a 68 kD enzyme which catalyses the synthesis of acetylcholine (Ach) from choline and acetyl coenzyme A. The human ChAT gene encodes two proteins, the 68 kD ChAT enzyme and a 27 kD protein immunologically related and coexpressed with ChAT in cholinergic neurons of the central nervous system. The smaller proteins may play a role in the regulation of ACh synthesis. ChAT is expressed in cholinergic neurons, the majority of the neurons in the nucleus basalis of Meynert, large neurones in the striatum (putamen and caudate nuclei), the majority of neurones in the pedunculo-pontine, hypoglossal, dorsal nucleus of vagus and subgroups of neurones in Roller,Ä111s and the medial olivary accessory nuclei. Prominent staining is observed in ribonucleoprotein, distributed at the periphery of large neurons of the nucleus basalis of Meynert, the motor neurons in the hypoglossal and ambiguus nuclei. NCL-ChAT does not label axons in the insular cortex of the internal capsule non-cholinergic structures, endothelial cells or microglia.

Aufreinigung: 200ug/ml of Ab produced in CHO cell mammalian-based expression system.

Immunogen: Prokaryotic recombinant protein corresponding to the C-terminal region of the human ChAT molecule.

Isotyp: IgG1, kappa

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C.Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Details zu Choline Acetyltransferase

Target: Choline Acetyltransferase (CHAT) (Choline O-Acetyltransferase (CHAT))

Andere Bezeichnung: Choline Acetyltransferase

Hintergrund:

CMS1A | CMS1A2 CMS6

Human appendix, colon, stomach or brain.

Molekulargewicht: 82kDa

Gen-ID: 1103, 410969

UniProt: P28329

Pathways: Skeletal Muscle Fiber Development