Choline Acetyltransferase Antikörper

Produktnummer ABIN6656077

Produktdetails anti-Choline Acetyltransferase Antikörper

Target: Choline Acetyltransferase (CHAT) (Choline O-Acetyltransferase (CHAT))

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser Choline Acetyltransferase Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC)

Kreuzreaktivität: Vogel, Huhn, Meerschweinchen, Human, Maus, Non-Human Primate

Kreuzreaktivität (Details): Cross reactivity with ChAT from other species has not been determined

Aufreinigung: Anti-ChAT antibody is directed against human ChAT protein. The antibody was affinity purified from monospecific antiserum by immunoaffinity purification. . However, reactivity is also expected from the following species based on 100% sequence homology: chicken, guinea pig, mouse, and non-human primates.

Immunogen:

Immunogen: Anti-ChAT Antibody was produced in rabbit by repeated immunizations with native choline acetyltransferase purified from human placenta.

Immunogen Type: Native Protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry Dilution: 1:100

Application Note: Anti-ChAT antibody is suitable for use in ELISA, Western Blotting and IHC. Specific conditions for reactivity should be optimized by the end user. Expect a ~ 68/70 kDa band corresponding to choline acetyltransferase protein by Western Blot in the appropriate lysate or tissue.

Western Blot Dilution: 1:100

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer:

Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 5 mg BSA

0.02 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening.  This product is stable at 4° C as an undiluted liquid.  For extended storage, aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. Dilute only prior to immediate use.

Referenzen für anti-Choline Acetyltransferase Antikörper (ABIN6656077)

Liu, Kim, Kim, Zhang, Bao, Denaxa, Lim, Kim, Liu, Wickersham, Pachnis, Hattar, Song, Brown, Blackshaw: "Lhx6-positive GABA-releasing neurons of the zona incerta promote sleep." in: Nature, Vol. 548, Issue 7669, pp. 582-587, (2017) (PubMed).

Details zu Choline Acetyltransferase

Target: Choline Acetyltransferase (CHAT) (Choline O-Acetyltransferase (CHAT))

Andere Bezeichnung: ChAT (Choline Acetyltransferase) (CHAT Produkte)

Synonyme: CHOACTASE antikoerper, CMS1A antikoerper, CMS1A2 antikoerper, ChoAcTase antikoerper, B230380D24Rik antikoerper, choline O-acetyltransferase antikoerper, choline O-acetyltransferase a antikoerper, choline acetyltransferase antikoerper, carnitine O-acetyltransferase antikoerper, CHAT antikoerper, chata antikoerper, Chat antikoerper, PGTG_00551 antikoerper, LOC100533322 antikoerper

Hintergrund:

Synonyms: acetyl CoA:choline O-acetyltransferase, CHAT, CHOACTase, Choline acetylase, choline acetyltransferase, Choline O-acetyltransferase, CLAT, CMS1A, CMS1A2

Background: ChAT antibody recognizes choline acetyltransferase which is a neuronal enzyme which catalyzes the reaction between Acetyl CoA and choline resulting in the formation of acetylcholine. ChAT is found primarily in cholinergic neurons making it a valuable marker for diseases associated with decreased cholinergic function such as Schizophrenia, Alzheimer disease (AD) and Down syndrome. Decreased choline acetyltransferase activity in particular has been shown in Schizophrenic subjects. It has furthermore been demonstrated that in patients with AD, there are significantly lower levels of cortical ChAT that correlate with severity of the disease as measured by loss of neuropsychological function. Anti-ChAT antibody is ideal for investigators involved in Cell Signaling, Neuroscience, Signal Transduction research.

Gene Name: CHAT

Gen-ID: 1103

UniProt: P28329

Pathways: Skeletal Muscle Fiber Development